【摘要】 目的：制备水痘-带状疱疹病毒（VZV）衣壳蛋白ORF41的单克隆抗体（mAbs），并对该蛋白性质进行初步研究。方法：截取VZV ORF41蛋白亲水性及免疫原性较优的片段，用大肠杆菌蛋白表达系统对相关区段进行表达并纯化，免疫BALB/c小鼠后，通过杂交瘤细胞技术制备并筛选出针对VZV ORF41的mAbs，并利用制备得到的mAbs进行ELISA、Western blot和免疫荧光实验，检测所得抗体的亲和性和特异性，同时分析VZV ORF41蛋白在细胞中的表达与分布特征。结果：本研究筛选到的VZV OR41 mAb 12C3具有较高的亲和性和特异性，可特异性识别在质粒转染及VZV感染细胞中表达的ORF41蛋白，且发现检测出的VZV ORF41蛋白在细胞质与细胞核中均有分布。结论：本研究获得针对VZV ORF41反应性、特异性良好的mAb，为进一步开展VZV ORF41蛋白功能和作用机制的研究提供基础。更多还原

【Abstract】 Objective:To prepare monoclonal antibodies(mAbs)target varicella-zoster virus(VZV)capsid protein ORF41and preliminarily study its properties. Methods:Fragments with good hydrophilicity and immunogenicity of VZV ORF41 protein were selected for production using an E.coli protein expression system and purified. After immunization in BALB/c mice, mAbs against VZV ORF41 were screened and prepared by hybridoma cell technology. With the mAbs, expression and distribution of VZV ORF41 protein in cells were analyzed by Western blot and immunofluorescence. Results: VZV OR41 mAb 12C3 screened in this study has high affinity and specificity, which could specifically recognize the ORF41 protein expressed in either plasmid-transfected or VZV-infected cells, and it is found that the VZV ORF41 protein is distributed in both cytoplasm and nucleus with the help of 12C3. Conclusion:In this study, an mAb with high reactivity and specificity against VZV ORF41 was obtained, which provided a basis for further studies on function and mechanism of VZV ORF41 protein.更多还原