【摘要】 目的 研究香烟暴露下前列腺基质细胞对上皮细胞的影响。方法 建立前列腺基质-上皮细胞共培养体系。观察不同条件下的基质细胞对上皮细胞增殖、胶原沉积、上皮-间质转化(EMT)的影响。收集前列腺基质细胞来源的外泌体，观察外泌体对上皮细胞的影响。通过高通量测序分析外泌体LncRNAs和mRNAs表达差异。结果 相对于空白对照，香烟暴露的基质细胞和基质来源外泌体均促进上皮细胞增殖、EMT和胶原沉积。测序和功能分析结果显示相对于空白对照，香烟暴露基质细胞来源的外泌体中有197个差异表达LncRNAs(158个上调，39个下调)和2 564个差异表达mRNAs(2 482个上调，82个下调)。差异表达的LncRNAs和mRNAs在调节细胞周期和增殖、细胞连接、纤维化等生物过程中发挥重要作用。结论 香烟暴露的前列腺基质细胞和其来源的外泌体能够促进上皮细胞增殖和胶原沉积。更多还原

【Abstract】 Objective To investigate the effects of cigarette smoke-exposed prostate stromal cells on epithelial cells.Methods The coculture systems of prostate stromal and epithelial cells were built.The effects of stromal cells on epithelial cell proliferation, collagen deposition and epithelial-mesenchymal transition(EMT) were observed under different conditions.The exosomes derived from prostate stromal cells were collected.The effects of exosomes on epithelial cells were evaluated.The expression difference of LncRNAs and mRNAs in exosomes was analyzed by high-throughput sequencing.Results Compared with blank control group, cigarette smoke-exposed prostate stromal cells and stromal cells-derived exosomes promoted epithelial cell proliferation, EMT and collagen deposition.Sequencing and functional analysis results showed that compared with blank control group, there were 197 differentially expressed lncRNAs(158 upregulated and 39 downregulated) and 2 564 differentially expressed mRNAs(2 482 upregulated and 82 downregulated) between exosomes-derived from cigarette smoke-exposed stromal cells and the vehicle.Differentially expressed LncRNAs and mRNAs played important roles in regulating cell cycle and proliferation, cell junction and fibrosis.Conclusions Cigarette smoke-exposed prostate stromal cells and their-derived exosomes could promote epithelial cell proliferation and collagen deposition.更多还原