【摘要】 目的：探讨复方沙棘籽油加速大鼠肛周脓肿术后创面修复的作用机制。方法：取制备成功肛周脓肿术后创面SD模型鼠共50只，随机分为5组，分别为模型组（G1，给予蒸馏水）；阳性对照组（G2，给予高锰酸钾）；复方沙棘籽油纳米乳化液低剂量治疗组（G3）；复方沙棘籽油纳米乳化液中剂量治疗组（G4）；复方沙棘籽油纳米乳化液高剂量治疗组（G5）；造模后各组实验大鼠创面用相应实验药物均匀涂抹，2次/d，连续给药14 d。观察治疗5 d、8 d、14 d后各组大鼠创面愈合情况，计算各组大鼠创面愈合率，分别取各组大鼠创面组织，应用免疫组化S-P法检测创面表皮生长因子（EGF）、磷酸化细胞外调节蛋白激酶（ERK）1/2、白细胞介素-8(IL-8)、前列腺素E2(PGE2)表达情况。结果：创面处理后5 d、8 d、14 d,G5组、G4组、G3组、G2组、G1组大鼠创面愈合率依次升高(均P <0.05)。术后14 d，大鼠创面IL-8表达水平为G2组<G5组<G4组<G3组<G1组(P <0.05);PEG2表达水平为G5组<G2组<G4组<G3组<G1组(P <0.05)。术后14 d，大鼠创面EGF、ERK1/2表达为G5组> G4组> G3组> G2组> G1组(均P <0.05)。结论：复方沙棘籽油纳米乳化液可有效促进大鼠肛周脓肿术后创面愈合，这可能与IL-8及PEG2炎症介质有关，还可能与EGF-ERK通路活化促进创面角质形成细胞、成纤维细胞生长、增殖、分化有关。更多还原

【Abstract】 Objective To study the effect of compound seabuckthorn seed oil on accelerating wound healing after perianal abscess surgery in rats. Methods A total of 50 SD model mice were used to successfully prepare postoperative wounds of perianal abscess. They were randomly divided into five groups: G1 model group(given distilled water); G2 positive control group(given potassium permanganate); G3 treatment group(low dose group of compound seabuckthorn seed oil nano-emulsion); G4 treatment group(middle dose group of compound seabuckthorn seed oil nano-emulsion); G5(high dose group of compound seabuckthorn seed oil nano-emulsion).After modeling, the wounds of experimental rats in each group were evenly smeared with corresponding experimental drugs twice a day for 14 days. Observe the wound healing of rats in each group after 5 d, 8 d and14 d of treatment, calculate the wound healing rate of rats in each group and take the wound tissue of rats in each group respectively, and analyze the expression of EGF, phosphorylated ERK1/2, IL-8 and PEG2 in the wound with immunohistochemical S-P method. Results The wound healing rate of rats in G5 group, G4 group,G3 group, G2 group and G1 group increased in turn at 5 d, 8 d and 14 d after wound treatment(all P <0.05). 14days after surgery, IL-8 expression in rat wounds G2<G5<G4<G3<G1(P <0.05); PEG2 expressed G5<G2<G4<G3<G1(P<0.05). 14 days after surgery,The expression of EGF and ERK1/2 in rat wounds was G5>G4>G3>G2 >G1(all P <0.05). Conclusion Compound seabuckthorn seed oil nano-emulsion can effectively promote wound healing after perianal abscess surgery in rats, which may be related to the inflammatory mediators of IL-8 and PEG2, and its activation of "EGF-ERK" pathway can promote the growth, proliferation and differentiation of wound keratinocytes and fibroblasts.更多还原