【摘要】 目的：探讨高迁移率族蛋白B1(HMGB-1)和帕金森病相关蛋白（DJ-1）在膀胱癌不同组织中的表达及其临床意义。方法：收集2005年1月—2015年1月天津医科大学第二医院和天津市南开医院收治的58例膀胱癌组织为实验组，其癌旁正常组织为对照组。应用免疫组织化学方法检测膀胱癌组织与癌旁组织中HMGB-1和DJ-1蛋白表达情况，分析二者的表达率与膀胱癌临床病理特征及患者预后的关系，Spearman相关分析膀胱癌中HMGB-1与DJ-1表达的相关性，Cox多因素回归分析膀胱癌患者预后的影响因素。结果：膀胱癌中HMGB-1、DJ-1阳性表达率高于癌旁组织，差异有统计学意义（P <0.05）;HMGB-1蛋白表达水平与病理分型、病理分级、淋巴结转移、TNM分期有关，肿瘤分化越差、淋巴结有转移、分期越高，HMGB-1蛋白表达水平越高（P <0.05）。DJ-1在58例膀胱癌中的表达与肿瘤数目、淋巴结转移、TNM分期有关，肿瘤多发、分期越高、淋巴结有转移，DJ-1蛋白表达水平越高（P <0.05）。Spearman相关分析结果显示，HMGB-1与DJ-1表达水平呈正相关（r=0.421,P <0.05）。HMGB-1和DJ-1蛋白表达水平较高的患者无病生存期低于HMGB-1和DJ-1蛋白低表达组（P <0.05）。Cox回归模型分析显示，HMGB-1和DJ-1阳性、腺癌、鳞癌、病理分级高、有淋巴结转移、TNM分期高是膀胱癌患者预后的独立危险因素。结论：检测膀胱癌组织中HMGB-1和DJ-1的表达水平有助于预测与评估膀胱癌的侵袭、转移及预后的风险，为膀胱癌围手术期的合理治疗方案选择提供重要依据。更多还原

【Abstract】 Objective To investigate the expression and clinical significance of high mobility group protein B1(HMGB-1) and Parkinson’s disease associated protein(DJ-1) in different tissues of bladder cancer. Methods From January 2005 to January 2015, 58 cases of bladder cancer treated in the Second Hospital of Tianjin Medical University and Tianjin Nankai Hospital were collected as the experimental group, and their adjacent normal tissues as the control group. Immunohistochemical method was used to detect the expression rates of HMGB-1 and DJ-1 proteins in bladder cancer tissues and adjacent tissues. The relationship between HMGB-1,DJ-1 and the clinicopathological features of bladder cancer and the survival of patients was analyzed. Spearman correlation analysis was used to analyze the correlation between HMGB-1 and DJ-1 expression in bladder cancer, and Cox multivariate analysis was used to analyze the prognostic factors of bladder cancer patients.Results The positive expression rates of HMGB-1and DJ-1 in bladder cancer were higher than those in adjacent tissues, and the difference was statistically significant(P <0.05). The expression level of HMGB-1 protein is related to pathological classification,histological differentiation, lymph node metastasis and TNM stage, and the worse the tumor differentiation,lymph node metastasis and stage, the higher the HMGB-1 expression(P <0.05). The expression of DJ-1 was related to the number of tumors, lymph node metastasis and TNM stage. The expression level of DJ-1 protein in multiple tumors, high TNM stage and lymph node metastasis group was significantly higher than that in single tumor, low stage and no local metastasis group(P <0.05). Spearman correlation analysis showed that the expression levels of HMGB-1 and DJ-1 were positively correlated(r=0.421, P <0.05). Patients with high expression of HMGB-1 and DJ-1 protein had a shorter diseasefree survival time than those with low expression of HMGB-1 and DJ-1 protein(P <0.05). Cox regression model analysis showed that HMGB-1, DJ-1, adenocarcinoma, squamous cell carcinoma, urothelial carcinoma,histological differentiation, lymph node metastasis and TNM stage can be used as independent risk factors to predict the prognosis of bladder cancer. Conclusion The detection of HMGB-1 and DJ-1 expression levels in bladder cancer is helpful to predict and evaluate the invasion, metastasis and prognosis risk of bladder cancer,and provides an important basis for the rational choice of perioperative treatment for bladder cancer.更多还原