【摘要】 目的 研究长链非编码RNA(lncRNA)分化非蛋白编码RNA(DANCR)靶向微小RNA(miR)-19a-3p对绝经后骨质疏松(PMOP)骨髓间充质干细胞(BMSCs)成骨分化的调控作用。方法 选择健康女性志愿者4例和PMOP患者4例，分离培养BMSCs; PMOP患者BMSCs进行分组，转染阴性对照(NC)siRNA、lncRNA DANCR siRNA、miR-NC抑制物、miR-19a-3p抑制物。检测lncRNA DANCR、miR-19a-3p、PTEN的表达水平，成骨诱导分化后检测茜素红染色的OD405水平、碱性磷酸酶(ALP)活力、骨钙素(OCN)及骨桥蛋白(OPN)的mRNA表达水平。结果 PMOP患者BMSCs中lncRNA DANCR、PTEN的表达水平高于健康女性，miR-19a-3p的表达水平及成骨诱导后的OD405水平、ALP活力、OCN及OPN的表达水平均低于健康女性。在PMOP患者BMSCs中，si-DANCR组的lncRNA DANCR、PTEN的表达水平低于si-NC组，成骨诱导后的OD405水平、ALP活力及miR-19a-3p、OCN、OPN的表达水平高于si-NC组；si-DANCR+miR-19a-3p抑制物组成骨诱导后的OD405水平、ALP活力及miR-19a-3p、OCN、OPN的表达水平低于si-DANCR+miR-NC抑制物组，PTEN的表达水平高于si-DANCR+miR-NC抑制物组。结论 LncRNA DANCR靶向miR-19a-3p调控PMOP患者BMSCs的成骨分化。更多还原

【Abstract】 Objective To study the regulatory effect of long non-coding RNA(lncRNA) differentiation antagonizing non-protein coding RNA(DANCR) targeting microRNA(miR)-19a-3p on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs) in postmenopausal osteoporosis(PMOP). Methods BMSCs were isolated from 4 healthy female volunteers and 4 PMOP patients and cultured. BMSCs of PMOP patients were grouped and transfected with negative control(NC) siRNA, lncRNA DANCR siRNA, miR-NC inhibitor, or miR-19a-3p inhibitor. The expression levels of lncRNA DANCR, miR-19a-3p, and PTEN were measured. OD 405 levels, vitality of alkaline phosphatase(ALP), mRNA expression levels of osteocalcin(OCN) and osteopontin(OPN) after osteogenic induction and differentiation were measured. Results The expression levels of lncRNA DANCR and PTEN in BMSCs of PMOP patients were higher than those in healthy women. The expression level of miR-19a-3p and OD 405 level, ALP viability, OCN and OPN expression levels after osteogenic induction were lower than those in healthy women. In BMSCs of PMOP patients, the expression levels of lncRNA DANCR and PTEN in the si-DANCR group were lower than those in the si-NC group, and the OD 405 levels, ALP viability, and the expression levels of miR-19a-3p, OCN, and OPN were higher than those in the si-NC group. The levels of OD 405 level and ALP viability and the expression levels of miR-19a-3p, OC, and OPN in si-DANCR+miR-19a-3p inhibitor group were lower than those of the si-DANCR+miR-NC inhibitor group. The expression level of PTEN was higher than that in the si-DANCR+miR-NC inhibitor group. Conclusion LncRNA DANCR targeting miR-19a-3p regulates the osteogenic differentiation of BMSCs in PMOP patients.更多还原