【摘要】 目的 优化鳖血冻干粉的制备工艺，为提高鳖血的服用便捷性和质量稳定性提供参考。方法 采用真空冷冻干燥技术制备鳖血冻干粉。以溶解度为考察指标，通过单因素实验和Box-Behnken响应面实验优化鳖血冻干粉的冷冻干燥工艺参数，并验证。结果 鳖血冻干粉最优冷冻干燥工艺为预冻时间4 h、干燥时间13 h(0℃前)、解析干燥温度25℃。3批按最优工艺制备的鳖血冻干粉的平均溶解度为95.72%(RSD=0.68%,n=3)，与理论溶解度（96.66%）的相对误差为-0.97%。结论 本研究优化的冷冻干燥工艺稳定可行，所制样品的溶解度较高。更多还原

【Abstract】 OBJECTIVE To optimize the preparation process of Soft-shelled turtle blood lyophilized powder(STBLP), and to provide a reference for improving the availability and quality stability of soft-shelled turtle blood(STB). METHODS STBLP was prepared with vacuum freeze-drying. Taking the solubility as the index, the preparation process parameters of STBLP were optimized by single factor experiment and Box-Behnken response surface method. RESULTS The optimal freeze-drying process for STBLP was obtained: pre-freezing time of 4 h, total drying time of 13 h(before at 0 ℃), and resolution drying temperature of 25 ℃. The average solubility of 3 batches of STBLP prepared according to the optimal process was 95.72%(RSD=0.68%, n=3), the relative error of which was-0.97% to the theoretical solubility(96.66%). CONCLUSIONS Optimized lyophilization process in this study are stable and feasible, the solubility of the prepared sample is high.更多还原