【摘要】 为了探究不同香菇配方培养料中胞外酶活性的变化规律以及对产量、子实体品质的影响，以香菇9608为材料，采用3种不同香菇培养料配方[配方1：栎木78%(w，后同)、麸皮20%、石灰1%、石膏1%；配方2：苹果木39%、栎木39%、麸皮20%、石灰1%、石膏1%；配方3：苹果木78%、麸皮20%、石灰1%、石膏1%]进行试验，检测了香菇不同生长时期羧甲基纤维素酶、半纤维素酶、淀粉酶和漆酶4种胞外酶的活性，香菇产量以及子实体中营养物质的含量。结果表明，配方2香菇羧甲基纤维素酶和淀粉酶活性在转色期和现蕾期较配方1和配方3高；配方2香菇总产量以及生物学效率最高，单袋平均产量1 126.1 g，比配方1、配方3分别增产6.87%、22.60%；配方2香菇子实体中氨基酸、蛋白质、维生素C含量均高于配方1和3。因此，使用栎木和苹果木混合培养料可提高香菇产量和品质。更多还原

【Abstract】 In order to explore the changes of extracellular enzyme activities in different formulations of Lentinus edodes culture materials and their effects on yield and fruiting body quality,In this paper,L.edodes 9608 was used as the material,and three different formulas of L.edodes culture materials were used(formula 1:oak 78%,bran 20%,lime 1%,gypsum1%;formula 2:apple wood 39%,oak 39%,Bran 20%,lime 1%,gypsum 1%;formula 3:apple wood 78%,bran 20%,lime1%,gypsum 1%)for testing.The activities of four extracellular enzymes,carboxymethyl cellulase,hemicellulase,amylase and laccase at different stages,the yield of shiitake mushroom and the content of nutrients in fruiting bodies were detected.The results showed that the activities of carboxymethyl cellulase and amylase in formula 2 were higher than those in formula 1 and formula 3 in mushroom color change period and budding periodcertain periods of growth;the total yield and biological efficiency of formula 2 were the highest,and the average yield per bag was 1 126.1g,which compared with formula 1 and formula 3,the yield was increased by 6.87% and 22.60% respectively;the content of amino acid,protein and Vc in the fruiting bodies of L.edodes in formula 2 were higher than that in formula 1 and 3.Therefore,the use of oak and apple wood mixed compost can improve the yield and quality of L.edodes.更多还原