【摘要】 目的 探讨哈萨克族2型糖尿病（type 2 diabetes mellitus,T2DM）发病率低于维吾尔族的可能原因，比较两个民族正常糖耐量人群肠道菌群、粪便及血浆代谢物的差异。方法 纳入哈萨克族正常糖耐量人（KNGT组）及维吾尔族正常糖耐量人（UNGT组）各8名，利用超高效液相色谱串联四极杆飞行时间质谱（UPLC-QTOF-MS/MS）对其粪便及血浆样本进行非靶向代谢组学检测，通过Illumina测序平台对其粪便样本进行宏基因组测序，对结果进行Wilcoxon检验及LEfSe差异分析。结果 非靶向代谢组学结果显示，KNGT组人群AICAR(5-氨基咪唑-4-甲酰胺核糖核苷)等5种粪便代谢物及1,7-Dimethylxanthine等5种血浆代谢物水平更高；宏基因组数据分析结果显示Dysgonomonadaceae等6种肠道细菌在UNGT组人群丰度较高，Alistipes putredinis等26种肠道细菌在KNGT组人群丰度较高，通过分析碳水化合物活性酶发现，两组人群糖苷水解酶和糖基转移酶的水平最高。结论 KNGT组与UNGT组人群中粪便、血浆代谢物及肠道细菌的差异可能是哈萨克族T2DM发病率较低的原因之一。更多还原

【Abstract】 Objective To explore the possible causes of low incidence rate of type 2 diabetes mellitus(T2DM)in Kazak than that in Uygur, compare the differences in intestinal bacteria, feces and plasma metabolites between the two peoples with normal glucose tolerance. Methods Eight Kazak normal glucose tolerant individuals(KNGT)and eight Uygur normal glucose tolerant individuals(UNGT)were included. Their feces and plasma samples were subjected to non-targeted metabonomic testing using ultra high performance liquid chromatography tandem quadrupole time of flight mass spectrometry(UPLC-QTOF-MS/MS); their feces samples were subjected to macrogenomic sequencing using the Illumina sequencing platform. The results were subjected to Wilcoxon test and LEfSe difference analysis. Results The results of non-targeted metabolomics showed that the levels of 5 fecal metabolites such as AICAR and 5 plasma metabolites such as 1,7-Dimethylxanthine in the KNGT group were higher. Macrogenomic data analysis showed that six intestinal bacteria such as Dysgonomonadaceae had a higher impact in the UNGT group, while 26 intestinal bacteria such as Alistipes putredinis had a higher impact in the KNGT group. Through analysis of carbohydrate active enzymes, it was found that the levels of glycoside hydrolase and glycosyltransferase in the two groups were the highest. Conclusion The differences in the feces,plasma metabolites and intestinal bacteria between KNGT group and UNGT group may be one of the reasons for the low incidence rate of T2DM in Kazak.更多还原