【摘要】 目的 探讨STZ诱导的DKD小鼠环状RNA(circRNA)的差异表达。方法 8只小鼠分为正常对照组（Con）、DKD组，每组各4只。采用circRNA芯片检测各组肾组织circRNA表达谱，筛选差异表达circRNA分子，采用实时荧光定量PCR验证差异表达circRNA分子在各组肾组织中的表达变化，采用TargetScan及miRanda软件预测差异表达circRNA可能作用的miRNA靶点。结果DKD组共筛选出177个差异表达circRNA（差异倍数≥1.2且P<0.05），其中62个circRNA上调，115个circRNA下调，表达上调前5的差异circRNA是mmu＿circRNA＿40538、mmu＿circRNA＿21098、mmu＿circRNA＿20027、mmu＿circRNA＿25333和mmu＿circRNA＿25074，表达下调前5的差异circRNA是mmu＿circRNA＿19015、mmu＿circRNA＿41646、mmu＿circRNA＿011174、mmu＿circRNA＿29639及mmu＿circRNA＿28869。与Con组比较，DKD组FBG、24 h UAlb、mmu＿circRNA＿25074表达升高（P<0.05或P<0.01），体重、mmu＿circRNA＿011174及mmu＿circRNA＿41646表达降低（P<0.05）。结论 STZ诱导的DKD小鼠肾组织中circRNA呈差异表达，为验证circRNA在DKD中的作用提供依据。更多还原

【Abstract】 【Objective】 To explore the expression of kidney circular RNA in mice with diabetic kidney disease(DKD)induced by STZ.MethodsA total of 8 mice were averagely divided into control group and DKD group. Different expression circRNAs were detected and screened by circRNA microchip. Then they were confirmed by quantitative real-time PCR in kidney tissue and compared between control and DKD mice.Finally,miRNA that can bind to circRNA was predicted by TargetScan & miRanda.ResultsA total of177 different circRNA were found in DKD group(fold change≥1. 2 and P<0. 05). Among them,62circRNAs were upregulated and 115 circRNAs were down-regulated. The top five upregulated circRNAs were mmu＿circRNA＿40538, mmu＿circRNA＿21098,mmu＿circRNA＿20027,mmu＿circRNA＿25333 and mmu＿circRNA＿25074. And the top five downregulated circRNAs were mmu＿circRNA＿19015,mmu＿circRNA＿41646,mmu＿circRNA＿011174,mmu＿circRNA＿29639 and mmu＿circRNA＿28869. Compared with Con group,FBG,24 h UAlb,and mmu＿circRNA＿2507 expression were increased(P<0. 05 or P<0. 01),while weight,mmu＿circRNA＿011174 and mmu＿circRNA＿41646 expression were decreased(P<0. 05)in DKD group.ConclusionThere were different expression circRNAs in kidney of STZ-induced DKD mice. It will provide the basis for further study on the possible involvement of circRNAs in the pathogenesis of DKD.更多还原