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体外受精胚胎培养过程中的微生物污染

Microbial contamination in the embryo culture process during IVF

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【作者】 韩伟刘霜刘军霞黄国宁

【Author】 HAN Wei;LIU Shuang;LIU Jun-xia;HUANG Guo-ning;Chongqing Health Center for Women and Children,Women and Children’s Hospital of Chongqing Medical University;

【通讯作者】 黄国宁;

【机构】 重庆市妇幼保健院重庆医科大学附属妇女儿童医院

【摘要】 胚胎培养过程偶有发生微生物污染的案例。微生物污染可能来自多个方面,如精液、卵泡液和体外操作环境等。常见的微生物污染包括细菌、霉菌和支原体。胚胎培养污染病例的报道较少,这使得许多中心认为这个问题可以忽略不计。文章就胚胎培养过程中常见微生物污染的来源、类型和镜下特点,污染后的处理措施及实验室如何避免污染的策略进行讨论,旨在为建立安全的体外胚胎培养体系提供参考。

【Abstract】 There have been occasional cases of microbial contamination in the process of embryo culture.Microbial contamination can come from various sources,such as semen,follicular fluid,and the in vitro environment. Common types of microbial contamination include bacteria,fungi,and chlamydia. There are few reports on cases of embryo culture contamination,which makes many centers believe that this issue can be ignored. This article discusses the sources,types,and microscopic characteristics of common microbial contamination in the embryo culture process,as well as the measures taken after contamination and strategies for laboratories to avoid contamination. The aim is to provide references for establishing a safe in vitro embryo culture system.

【基金】 重庆市科卫联合医学科研项目(2021MSXM108)
  • 【文献出处】 中国实用妇科与产科杂志 ,Chinese Journal of Practical Gynecology and Obstetrics , 编辑部邮箱 ,2023年10期
  • 【分类号】R714.8
  • 【下载频次】5
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