【摘要】 为了解非洲猪瘟病毒（African swine fever virus,ASFV）在规模养殖场传播媒介中的污染状况，2020—2021年在辽宁省部分规模猪场采集人员、物资、车辆和环境类样品共269 810份，采用荧光PCR方法进行ASFV核酸检测。结果显示：被检规模猪场人员、物资、车辆和环境类样品ASFV核酸阳性检出率分别为0.73%、0.49%、0.36%和0.05%，其中入场员工、食品物资、拉猪车和外周场地样品ASFV核酸阳性检出率在同类样品中最高；蔬菜样品在物资类样品中ASFV核酸阳性检出率最高，为0.13%；驾驶室和车身样品在车辆类样品中ASFV核酸阳性检出率较高，分别为0.11%和0.10%。结果表明，规模猪场入场员工、蔬菜等食品类物资、车辆驾驶室和车身都是ASFV污染高风险点，寒冷的11月至次年4月是ASFV污染发生的高危时间段。建议重点对高风险时段的高风险人员、物资、车辆和环境有针对性制定精准的消毒等综合防控措施，以有效降低ASFV污染风险。更多还原

【Abstract】 In order to investigate African swine fever virus(ASFV)carried by the vectors in large-scale swine farms,a total of 269 810 samples from workers,materials,vehicles and environment were collected from some farms in Liaoning Province from 2020 to 2021,and detected for ASFV nucleic acids by fluorescent PCR. The results showed that the positive detection rates were 0.73%,0.49%,0.36% and 0.05%,respectively from the above samples,highest in the samples from entry workers,food supplies,vehicles for transporting pigs and surrounding environment as compared to those under the same category,highest(0.13%)in vegetable samples under the category of materials,and highest in driving cab and vehicle body samples under the same category,0.11% and 0.10%,respectively.In conclusion,entry workers,vegetables and other food materials,driving cabs and vehicle bodies were at high risk of ASFV contamination,with a period at high risk from November when it was cold to next April. It was thus recommended to effectively reduce the risk of ASFV contamination through specifically developing comprehensive prevention and control measures including disinfection for the workers,materials,vehicles and environment at high risk during the above period.更多还原