【摘要】 以柠条为原料，设置对照组(无添加剂)和添加植物乳杆菌处理组，测定厌氧发酵14 d、56 d的营养成分、发酵品质、pH值以及发酵56 d青贮微生物种群，分析植物乳杆菌对柠条青贮的影响。结果表明：青贮14 d、56 d,添加植物乳杆菌处理组与对照组相比，水溶性碳水化合物、乳酸含量、乳酸/乙酸显著提高(P<0.05);酸性洗涤纤维含量显著降低，粗蛋白质含量无显著性差异。青贮14 d,两组干物质含量无显著性差异，而青贮56 d,添加植物乳杆菌处理组干物质含量比对照组降低了4.58%(P<0.05)。青贮56 d,柠条青贮微生物群落在门水平上，添加植物乳杆菌处理组厚壁菌门丰度比对照组增加了30.84%(P<0.05),而变形菌门丰度降低了93.92%(P<0.05)。在属水平上，与对照组相比，添加植物乳杆菌处理组乳杆菌属丰度显著增加了140.74%(P<0.05),肠杆菌属、肠球菌属，片球菌属丰度显著下降(P<0.05),魏斯氏菌属丰度下降，但差异不显著。综上，添加植物乳杆菌能提高柠条青贮饲料的营养成分和发酵品质，同时降低不良微生物肠杆菌属的丰度，增加有益菌乳杆菌属的丰度。因此，在生产实践中，该植物乳杆菌可作为柠条青贮的备选菌种。更多还原

【Abstract】 This study aimed to explore the effects of Lactobacillus plantarum supplementation on Caragana silage by determining the nutrient composition, fermentation quality, and pH value of anaerobic fermentation at 14 d and 56 d, and the microbial population of silage at 56 d of fermentation. The results showed that the contents of water-soluble carbohydrate and lactic acid, and ratio of lactic acid/acetic acid were significantly higher(P<0.05) in the Lactobacillus plantarum group compared with the control group at 14 d and 56 d of fermentation. While the acidic detergent fiber content was significantly lower in the Lactobacillus plantarum group and crude protein content was similar between the two groups. The dry matter content of the Lactobacillus plantarum group at 56 d of fermentation was decreased by 4.58% compared to the control group(P<0.05), while no significant difference of dry matter content was observed between the two groups at 14 d of fermentation. At the phylum level, the abundances of Firmicutes and Proteobacteria in the Lactobacillus plantarum group at 56 d of fermentation was increased and decreased by 30.84% and 93.92%, respectively, compared with the control group(P<0.05). At the genus level, the abundance of Lactobacillus in the Lactobacillus plantarum group was increased by 140.74%(P<0.05) compared with the control group, while the abundances of Enterobacter, Enterococcus, and Pediococcus were significantly decreased(P<0.05). In conclusion, the addition of Lactobacillus plantarum could improve the nutrient composition and fermentation quality of Caragana silage, reduce the abundance of undesirable microorganisms Enterobacter, and increase the abundance of beneficial bacteria Lactobacillus. Therefore, Lactobacillus plantarum can be used as an alternative bacterial strain for Caragana silage production.更多还原