【摘要】 为明确小麦条锈菌生理小种的构成及其多态性分布，以2023年从湖北省10个市采集分离的113份条锈菌标样为材料，采用19个鉴别寄主进行了生理小种鉴定和群体毒性结构分析，共鉴定出Hybrid 46、水源11、贵农22三致病类群的20个已知生理小种。其中，Hybrid 46类群为2023年湖北省小麦条锈菌的优势致病类群；CYR32和CYR33为优势生理小种，发生频率分别为33.63%和16.81%。群体毒性多态性分析表明，在物种水平上，湖北省小麦条锈菌毒性多样性较为丰富，Nei’s基因多样性指数为0.198,Shannon’s信息指数为0.316；在群体水平上，不同区域间毒性多样性存在一定差异，其中黄石和十堰的条锈菌毒性多样性水平最高，武汉的最低，但地理距离并不是导致条锈菌群体毒性多样性的主要原因。UPGMA聚类显示，10个采集地小麦条锈菌群体的毒性遗传相似系数为0.92～0.99，不同群体间毒性结构较相似，交流广泛。本研究结果可为湖北省小麦抗锈育种和通过抗病品种合理布局防控条锈病提供科学依据。更多还原

【Abstract】 This study aimed to identify the races composition and polymorphism of Puccinia striiformis f. sp. tritici(Pst) by analyzing 113 isolates collected from ten counties(cities) in Hubei Province in 2023. The population virulence structure was determined using 19 differential hosts in China. The results showed that 20 known Pst races in Hybrid 46, Suwon 11,and Guinong 22 groups were identified from 113 isolates. The Hybrid 46 group was found to be the dominant pathogenic group of Pst in Hubei province in 2023. Among these races, CYR32 and CYR33 were the most prevalent, accounting for33.63% and 16.81%, respectively. Population virulence polymorphism analysis revealed that Pst had high virulence diversity in Hubei province at the species level, with Nei’s gene diversity index(H) of 0.198 and Shannon’s information index(I) of 0.316. At the population level, there were differences in virulence diversity of Pst among different region. Pst in Huangshi and Shiyan had the highest virulence diversity, while that in Wuhan was the lowest. However, geographic distance was not the main reason for the population virulence diversity of Pst. The UPGMA cluster analysis showed that the genetic similarity coefficients of virulence of 10 Pst populations in Hubei province ranged from 0.92 to 0.99, indicating that the virulence structure of different populations was similar and the communication among different groups was extensive. These results provide a scientific basis for breeding wheat with stripe rust resistance in Hubei province and for controlling of wheat stripe rust through the rational distribution of resistant varieties.更多还原