【摘要】 目的 基于液相色谱-质谱联用（LC-MS）技术从脂质组学角度探讨幽门螺杆菌（Helicobacter pylori，简称H. pylori）与胃组织病变损伤的关系。方法 收集胃疾病患者31例，对其胃窦、胃体黏膜组织块进行病理检测与H. pylori培养与细菌鉴定，采用免疫印迹法进行血清H. pylori抗体分型。用LC-MS法检测H. pylori菌体的脂质成分，采用主成分分析和正交偏最小二乘法判别方法分析两组的脂质代谢谱差异，筛选差异性脂质代谢物。结果 病理学诊断为炎症11例（无异变组）、萎缩/化生及肿瘤20例（异变组）。H. pylori血清抗体分型为抗体Ⅰ型、抗体Ⅱ型、阴性。与抗体Ⅱ型组比较，抗体Ⅰ型组HE染色可见胃壁细胞肿大、坏死，伴有明显的炎症浸润同时伴肠异生、萎缩等；H. pylori共培养出27例；与无异变组比较，发现异变组有9个明显差异的脂质，其中胆固醇酯（CE）显著升高，具有统计学差异（P <0.05）。与化生组比较，发现肿瘤组的三酰甘油（TAG）与CE明显升高，具有统计学差异（P <0.05）。结论 脂质组学研究揭示幽门螺杆菌致胃组织病变损伤与菌体脂质CE/TAG有关。更多还原

【Abstract】 Objective To explore the relationship between Helicobacter pylori(H. pylori)-induced gastric tissue lesions and damage from the perspective of lipidomics based on Liquid Chromatography Mass Spectrometry(LC-MS) technology research. Methods Thirty-one patients with gastric diseases were collected. Pathological examination, H. pylori culture and bacterial identification were performed on the gastric antrum and gastric corpus mucosal tissue blocks, and the serum H. pylori antibody was typed by western blotting. The lipid composition of H. pylori cells was detected by LC-MS method, and the differences in lipid metabolism profiles of the two groups were analyzed by principal component analysis and orthogonal partial least squares discriminant method, and differential lipid metabolites were screened. Results Pathological diagnosis was inflammation in 11 cases(no mutation group), atrophy/metaplasia, and tumor in 20 cases(mutation group). H. pylori serotypes were classified as antibody type I, antibody type II, and negative. Compared with the antibody type II group, HE staining in the antibody type I group showed swelling and necrosis of gastric parietal cells, accompanied by obvious inflammatory infiltration and intestinal dysplasia, atrophy, etc.; 27 cases of H. pylori were co-cultured; by comparison, it was found that there were 9 significantly different lipids in the mutant group, among which cholesterol ester(CE)was significantly increased, with a statistical difference(P<0.05). Compared with the metaplasia group, it was found that the triacylglycerol(TAG)and CE of the tumor group were significantly increased, with a statistical difference(P<0.05). Conclusion Lipidomics study revealed that H. pylori gastric tissue lesions and damage are significantly different from those in the tumor group. Bacterial lipid CE/TAG related.更多还原