【摘要】 目的 探讨2型糖尿病（type 2 diabetes mellitus, T2DM）大鼠发病前后肠道菌群的变化。方法 8周龄雄性SD大鼠按照随机数字表法分为对照组（C组）和T2DM组（D组）,每组各5只。C组持续普通喂养，D组以高脂喂养4周（T₀期）后单次腹腔注射链脲佐菌素（streptozotocin, STZ）诱导T2DM（T₁期）。在T₀和T₁期收集粪便样本提取DNA进行16S rDNA-V3V4区测序，完成肠道菌群定量分析及优势菌群代谢功能预测。结果 Chao1和ACE指数在T₁期D组中下降。在门水平，T₀期D组大鼠厚壁菌门、放线菌门和变形菌门丰度增多，拟杆菌门减少；T₁期D组大鼠变形菌门更丰富。在属水平，T₀期D组大鼠乳杆菌属和普雷沃菌丰度下降，以颤螺菌属、克里斯滕森菌属和优杆菌属为核心的菌群增多；T₁期D组普雷沃菌属减少，以副拟杆菌属和优杆菌属为主的菌群增多。代谢预测示核酸和多糖代谢在C组富集，碳水化合物代谢在D组富集。结论 肠道菌群可能参与高脂联合STZ诱导T2DM大鼠的早期病理生理过程。更多还原

【Abstract】 Objective To explore the change of intestinal flora before and after onset of type 2 diabetes mellitus（T2DM） in rats. Methods Eight-week-old male SD rats were divided into control group（group C, n=5） and T2DM group（group D, n=5） by random number table method. The rats in group C were given continuous ordinary feeding, the rats in group D were fed with high-fat diet for 4 weeks（T₀stage） and followed by intraperitoneal injection of streptozotocin（STZ）（T₁stage）. Fecal samples were collected at stage T₀ and T₁ to extract DNA for sequencing of 16S rDNA-V3V4 region, and to complete quantitative analysis of intestinal flora and prediction of metabolic function of dominant flora. Results The Chao1 and ACE index decreased in group D at T₁stage. At the phylum level, the abundance of Firmicutes, Actinobacteria and Proteobacteria increased, and the Bacteroidetes decreased in the group D at T₀stage. Proteobacteria were more abundant in group D rats at T₁stage. At the genus level, the abundance of Lactobacillus and Prevotella decreased, while the flora of Oscillospira, Christensenella and Eubacterium increased in group D at the T₀stage. The nubmer of Prevotella decreased, while the number of bacteria mainly composed of Parabacteroides and Eubacterium increased in group D at T₁stage. Nucleotide metabolism and glycan biosynthesis and metabolism were enriched in group C, but carbohydrate metabolism was enriched in group D. ConclusionIntestinal flora may be involved in the early pathophysiological process of T2DM rats induced by high-fat diet combined with STZ.更多还原