【摘要】 目的 探讨细胞因子信号转导抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)在儿童过敏性紫癜(Henoch-Schonlein purpura, HSP)外周血中的表达特点，旨在分析其表达在HSP中的作用，为今后临床工作提供新的思路。方法应用反转录-聚合酶链反应检测在新疆医科大学第一附属医院儿科中心首次就诊的25例HSP患儿、25例HSPN患儿及健康对照儿童25例的外周血单个核细胞中的SOCS-3mRNA的表达水平，然后运用合适的统计学分析方法对相关数据进行分析和处理。结果 通过对3组儿童外周血中SOCS-3mRNA表达水平的比较发现HSPN组SOCS-3mRNA相对表达量高于HSP组及对照组，差异有统计学意义(H=19.06,P<0.05)。在25例HSP儿童各临床分型外周血SOCS-3mRNA相对表达量的分布特点分析比较中发现，差异无统计学意义(H=0.55,P>0.05)。在对25例HSPN儿童起病时各临床分型外周血SOCS-3mRNA相对表达量的比较中发现，差异有统计学意义(H=9.13,P<0.05),且在进一步的组间比较中发现腹型HSP儿童的SOCS-3mRNA相对表达量的分布较其余几种类型更高。在对HSP组与HSPN组儿童起病时各临床分型外周血SOCS-3mRNA相对表达量的比较中发现腹型及混合型紫癜的分布具有差异性(H=2.32,P<0.05;H=-2.04,P<0.05)。结论 SOCS-3参与了HSP的发病并可能与肾脏损害的发生有关。以胃肠道症状起病的HSP患儿后期可能更易出现肾脏损害。更多还原

【Abstract】 Objective To explore the expression characteristics of cytokine signaling-3 [suppressor of cytokine signaling-3(SOCS-3)] in the peripheral blood of Henoch-Schonlein purpura(HSP), and to analyze the role of its expression in HSP and provide new ideas for future clinical work.Methods The reverse transcription-polymerase chain reaction was used to detect the SOCS-3mRNA expression levels in peripheral blood mononuclear cells of 25 children with HSP, 25 children with Henoch-Schonlein purpura nephritis(HSPN) and 25healthy control children first treated in the Pediatric Center of the First Affiliated Hospital of Xinjiang Medical University. The relevant data were analyzed and processed.Results Results from the comparison of SOCS-3mRNA expression levels in the peripheral blood of the three groups showed that the relative expression of SOCS-3mRNA in HSPN group was significantly higher than that in HSP and control groups(H=19.06, P<0.05). There was no significant difference in the distribution and characteristics of the relative peripheral blood SOCS-3mRNA expression in 25 HSP children(H=0.55, P>0.05). Significant difference was found in the comparison of peripheral SOCS-3mRNA expression in 25 children with HSPN onset(H=9.13, P<0.05), and the distribution of SOCS-3mRNA expression in the HSP children with abdominal HSP was higher than in other types. Comparison of the relative SOCS expression of peripheral S m R N A between HSP and HSPN children(H=2.32, P<0.05; H=-2.04, P<0.05).Conclusion SOCS-3 is involved in the pathogenesis of HSP and may be related to the occurrence of renal damage. Children with HSP starting with gastrointestinal symptoms may be more likely to develop renal damage in the later stages.更多还原