【摘要】 目的 探究盘状结构域受体1(DDR1)对结直肠癌侵袭、上皮间质转化（EMT）的影响及机制。方法 收集2018年1月至2021年1月在日照市人民医院进行外科手术肿瘤切除治疗的60例结直肠癌癌组织及其癌旁组织。免疫组化检测结直肠癌组织和癌旁组织中DDR1蛋白表达水平。体外培养人结肠上皮细胞和结直肠癌细胞，Western blot检测DDR1蛋白表达水平。随后结直肠癌上皮细胞分为转染DDR1阴性干扰片段组（DDR1-NC）、转染DDR1干扰片段组（DDR1-siRNA）、过表达DDR1无关片段组（OVE-NC）和过表达DDR1组（OVE-DDR1）,Western blot检测DDR1、IL-6、p-STAT3和EMT相关蛋白表达变化。Trasnswell检测结直肠癌细胞侵袭情况。结果 与癌旁组织相比，结直肠癌组织DDR1蛋白阳性表达率明显增加，差异有统计学意义（χ~2=20.979,P<0.05）。与人结肠上皮细胞相比，结直肠癌细胞DDR1蛋白表达明显增加差异有统计学意义（t=2.970、8.398、4.977、5.253,P<0.05）。与DDR1-NC组相比，DDR1-siRNA组细胞DDR1、IL-6、p-STAT3蛋白表达降低，差异有统计学意义（t=3.533、4.021、3.864,P<0.05），结直肠癌细胞侵袭能力降低，差异有统计学意义（t=6.695,P<0.05）、EMT进程被抑制。与OVE-NC组相比，OVE-DDR1组细胞DDR1、IL-6、p-STAT3蛋白表达升高，差异有统计学意义（t=4.752、6.759、4.896,P<0.05），结直肠癌细胞侵袭能力增加，差异有统计学意义（t=4.181,P<0.05），促进EMT进程。结论DDR1在结直肠癌细胞中表达升高，其能够通过调控IL-6/STAT3信号促进结直肠癌细胞侵袭和EMT。更多还原

【Abstract】 Objective To investigate the effect and mechanism of discoid domain receptor 1(DDR1)on invasive epithelial-mesenchymal transition(EMT)in colorectal cancer. Methods From January 2018 to January 2021,60 cases of colorectal cancer and their paracancerous tissues underwent surgical tumor resection in Rizhao People’s Hospital were collected. Immunohistochemistry was used to detect the expression level of DDR1 protein in colorectal cancer and adjacent tissues. Human colon epithelial cells and colorectal cancer cells were cultured in vitro,and the expression level of DDR1 protein was detected by Western blot.Colorectal cancer epithelial cells were then divided into DDR1-negative interfering fragment group(DDR1-NC),DDR1 interfering fragment transfecting group(DDR1-siRNA),DDR1-overexpressing irrelevant fragment group(OVE-NC),and DDR1-overexpressing group( OVE-DDR1),Western blot was used to detect the expression changes of DDR1,IL-6,STAT3 and EMT-related proteins. The invasion of colorectal cancer cells was detected by Trasnswell. Results Compared with adjacent tissues,the expression of DDR1 protein in colorectal cancer tissues was significantly increased(P<0.05). Compared with human colon epithelial cells,the expression of DDR1 protein in colorectal cancer cells was significantly increased(P<0.05). Compared with DDR1-NC group,the expression of DDR1,IL-6 and p-STAT3 proteins in DDR1-siRNA group decreased(P<0.05),the invasive ability of colorectal cancer cells was decreased(P<0.05),and the EMT process was inhibited. Compared with the OVE-NC group,the expression of DDR1,IL-6 and p-STAT3 proteins were increased(P<0.05),the invasive ability of colorectal cancer cells was increased(P<0.05),and the EMT process was promoted in the OVE-DDR1 group(P<0.05). Conclusion The expression of DDR1 is increased in colorectal cancer cells,and it can promote colorectal cancer cell invasion and EMT by regulating IL-6/STAT3 signaling.更多还原