【摘要】 目的 探讨miR-134抑制食管鳞状细胞癌生长和转移的分子机制。方法 将人ESCC细胞系（TE-10）细胞分为miR-NC组和miR-134组，miR-NC组转染阴性mimic,miR-134组转染miR-134 mimic;qPCR法检测两组细胞中miR-134的表达，CCK8法检测两组细胞增殖能力，细胞划痕实验检测两组细胞迁移能力，Transwell检测两组细胞侵袭能力，免疫印迹法检测两组细胞内基质金属蛋白酶3(MMP3)和表皮因子生长受体（EGFR）蛋白的表达。结果 qPCR结果显示，miR-134组细胞miR-134表达量高于miR-NC组（P<0.05）;CCK8实验、细胞划痕实验和Transwell实验结果显示，与miR-NC组相比，mi R-134组细胞相对增殖能力降低了12.72%(P<0.05)，细胞迁移率下降了19.78%(P<0.05)，细胞侵袭率降低了44.47%(P<0.05)；免疫印迹法结果显示，miR-134组细胞MMP3和EGFR蛋白的表达均低于miR-NC组（P<0.05）。结论 miR-134可能通过调控MMP3和EGFR抑制TE-10细胞的增殖、迁移和侵袭。更多还原

【Abstract】 Objective To investigate the molecular mechanism of miR-134 inhibiting the growth and metastasis of esophageal squamous cell carcinoma.Methods TE-10 cells were divided into miR-NC group and miR-134 group. The miR-NC group was transfected with negative mimic, and the miR-134group was transfected with miR-134 mimic. The expression of miR-134 in the two groups was detected by q PCR. The proliferation ability of the two groups was detected by CCK8. The migration ability of the two groups was detected by cell scratch test. The invasion ability of the two groups was detected by Transwell. The expression of matrix metalloproteinase 3(MMP3) and epidermal growth factor receptor( EGFR) protein in the two groups was detected by Western blotting.Results The results of qPCR showed that the expression of miR-134 in miR-134 group was higher than that in miR-NC group(P<0.05). The results of CCK8 assay, cell scratch assay and Transwell assay showed that compared with miR-NC group, the relative proliferation ability of miR-134 group decreased by 12.72%(P<0.05), the cell migration rate decreased by 19.78%(P<0.05), and the cell invasion rate decreased by44.47%(P<0.05). Western blot results showed that the expression of MMP3 and EGFR protein in miR-134 group was lower than that in miR-NC group(P<0.05).Conclusion miR-134 may inhibit the proliferation, migration and invasion of TE-10 cells by regulating MMP3 and EGFR.更多还原