【摘要】 目的 探讨Discs大同源物关联蛋白5(DLGAP5)表达在肾透明细胞癌（KIRC）中的作用。方法 基于癌症基因组图谱（TCGA）分析KIRC中DLGAP5的表达情况与临床参数之间的相关性。应用比例风险（Cox）回归模型和Kaplan-Meier(K-M)分析评估了DLGAP5在KIRC患者中的预后价值。CIBERSORT分析DLGAP5和CD8~+T细胞之间的相关性，基因集富集分析（GSEA）方法筛选和DLGAP5表达相关的生物学途径。结果 与正常肾脏组织比较，KIRC中DLGAP5的表达呈显著上调。其高表达情况与肿瘤组织的分化程度、病理分期、复发和死亡均显著相关。K-M分析结果表明，DLGAP5高表达与KIRC患者的不良总生存期（OS）和无进展生存期（PFS）相关，且DLGAP5是OS和PFS的独立预后因子。免疫微环境分析表明，DLGAP5的表达量与多种肿瘤免疫细胞相关，尤其是CD8~+T细胞。DLGAP5表达在“细胞周期”“泛素介导的蛋白水解”“蛋白酶体通路”和“T细胞受体信号通路”上显著富集。药敏分析结果显示，高危组患者对阿昔替尼、舒尼替尼更敏感。结论 DLGAP5是KIRC的一种潜在生物标志物，与KIRC的诊断与预后显著相关，这将为临床的诊断与治疗提供新的研究方向。更多还原

【Abstract】 Objective To investigate the role of discs large homolog associated protein 5(DLGAP5) expression in renal clear cell carcinoma(KIRC).Methods The correlation between the expression of DLGAP5 in KIRC and clinical parameters was analyzed based on The Cancer Genome Atlas(TCGA). The prognostic value of DLGAP5 in KIRC patients was evaluated by proportional risk(Cox) regression model and Kaplan-Meier(K-M)analysis. CIBERSORT was used to analyze the correlation between DLGAP5 and CD8~+T cells. Gene set enrichment analysis(GSEA) was used to screen the biological pathways related to DLGAP5 expression.Results Compared with normal kidney tissues, the expression of DLGAP5 in KIRC was significantly up-regulated. Its high expression was significantly correlated with tumor differentiation, pathological stage, recurrence and death. K-M analysis showed that high expression of DLGAP5 was associated with poor overall survival(OS) and progression-free survival(PFS) in KIRC patients,and DLGAP5 was an independent prognostic factor for OS and PFS. Immune microenvironment analysis showed that the expression of DLGAP5 was associated with a variety of tumor immune cells, especially CD8~+T cells. DLGAP5 expression was significantly enriched in “cell cycle”“ubiquitinmediated proteolysis”“ proteasome pathway”and“T cell receptor signaling pathway”. The results of drug sensitivity analysis showed that patients in the high-risk group were more sensitive to axitinib and sunitinib.Conclusion DLGAP5 is a potential biomarker of KIRC, which is significantly related to the diagnosis and prognosis of KIRC, and will provide a new research direction for clinical diagnosis and treatment.更多还原