【摘要】 目的 观察桑寄生水提物灌胃对血管性痴呆（VD）大鼠学习记忆能力的影响，并探讨其作用机制。方法将健康雄性SD大鼠随机分为假手术组、模型组、桑寄生低剂量组（桑低组）和桑寄生高剂量组（桑高组），每组各8只。模型组、桑低组、桑高组大鼠利用双侧颈总动脉反复缺血再灌注加双侧永久结扎法建立VD模型，假手术组大鼠只分离不结扎动脉，造模后第2天，桑低组、桑高组分别灌胃给予1 g/kg和2 g/kg桑寄生水提物，其他组给予等量蒸馏水，每天1次，连续4周。采用Morris水迷宫实验观察各组大鼠的学习记忆能力；采用WST-1法测定海马组织中超氧化物歧化酶（SOD）活力，采用TBA法测定丙二醛（MDA）含量，采用酶标仪检测乙酰胆碱（Ach）含量。结果 在Morris水迷宫定位航行实验中，与假手术组相比，模型组从第3天开始逃避潜伏期延长（P均<0.05）；与模型组相比，桑低组从第4天、桑高组从第3天开始大鼠逃避潜伏期降低（P均<0.05）。在空间搜索实验中，模型组比假手术组大鼠的穿台次数减少、游行速度减慢（P均<0.01）；与模型组相比，桑低组、桑高组大鼠穿台次数增多，游行速度加快（P均<0.05）。与假手术组相比，模型组的SOD活力降低、MDA含量升高、Ach含量降低（P均<0.05）。与模型组相比，桑高组的SOD活力升高（P<0.05），桑低组与桑高组MDA含量降低、Ach含量增加（P均<0.05）。与桑低组相比，桑高组的SOD活力高（P<0.01），但MDA含量、Ach含量两组相比差异无统计学意义。结论 桑寄生水提物对VD大鼠的学习记忆能力有改善作用，其作用机制可能与桑寄生可提高海马组织中SOD活力、增加Ach含量、降低MDA含量有关。更多还原

【Abstract】 Objective To investigate the protective effect of Herba Taxilli on learning and memory function in vascular dementia(VD) rats, and to explore its mechanism. Methods SD rats were randomly divided into sham-operated group, model group, low dose group and high dose group, with 8 rats in each group. VD rat model was established by bilateral common carotid artery repeated ischemia-reperfusion and permanent bilateral artery occlusion. The model group, low and high dose group were all VD rats. Rats in the sham-operated group were isolated without ligating their arteries. On the second day after modeling, the rats in the low and high dose groups were given 1 g/kg and 2 g/kg of Herba Taxilli by gavage. Other groups were given equal volume of distilled water, once a day for 4 consecutive weeks. The learning and memory function was tested by Morris water maze. The activity of superoxide dismutase(SOD) and the content of malondialdehyde(MDA) in hippocampus were measured by WST-1 and TBA method, the exprssion of acetylcholine(Ach) was detected by microplate reader. Results In Morris water maze, compared with the sham-operated group, the escape latency of the model group was prolonged from the 3rd day(all P< 0. 05). Compared with the model group, the escape latency of rats in the low and high dose of Herba Taxilli groups was decreased from the 4th and 3rd day(all P<0. 05). In Spatial probe test, the model group had lower crossing platform times and slower movement speed than the sham-operated group(all P<0. 01). Compared with the model group, crossing platform times was increased and the speed of walking was accelerated in the low and high dose of Herba Taxilli groups(all P<0. 05). Compared with the sham-operated group, the activity of SOD and the expression of Ach in the model group were decreased, the content of MDA was increased(all P<0. 05). Compared with model group, the activity of SOD of the high dose of Herba Taxilli group increased(P<0. 05), while the MDA content decreased and the Ach content increased(all P<0. 05) in the low and high dose of Herba Taxilli groups. Compared with the low dose of Herba Taxilli group, the high dose of Herba Taxilli group had higher SOD activity(P<0. 01), but there was no statistically significant difference in MDA content and Ach content between the two groups. Conclusion Herba Taxilli can significantly improve learning and memory function of VD rats which may be related to improve of the activity of SOD and the content of MDA and reduce the level of Ach.更多还原