【摘要】 目的：探究KEAP1-NFE2L2基因相对拷贝数在肺癌发生发展机制中的作用，并分析KEAP1-NFE2L2基因相对拷贝数与患者的临床病理特征的关系。方法：采用实时荧光定量PCR方法检测肺癌患者和健康志愿者的血液样本中KEAP1-NFE2L2基因相对拷贝数和mRNA表达，分析它们在肺癌患者和健康志愿者之间的差异，同时利用Pearson相关性分析KEAP1-NFE2L2基因相对拷贝数和临床病理特征的关系。结果：肺癌患者NFE2L2以及MYC的相对拷贝数高于健康志愿者，差异有统计学意义(P<0.005)。肺癌患者中NFE2L2相对拷贝数与CYFRA21呈明显正相关(P=0.031),MYC拷贝数与KEAP1拷贝数呈正相关(P=0.008)。结论：MYC和NFE2L2的基因拷贝数可能和肺癌的发生和发展机制有关。更多还原

【Abstract】 Objective:To investigate the role of KEAP1-NFE2L2 gene relative copy number in the mechanism of lung cancer development and to analyze the relationship between KEAP1-NFE2L2 gene relative copy number and the clinicopathological characteristics of patients.Methods:The relative copy number and mRNA expression of KEAP1-NFE2L2 gene in blood samples from lung cancer patients and healthy volunteers were measured by real-time fluorescence quantitative PCR, and their differences between lung cancer patients and healthy volunteers were analyzed, while the relationship between the relative copy number of KEAP1-NFE2L2 gene and clinicopathological characteristics was analyzed using Pearson correlation.Results:The relative copy numbers of NFE2L2 as well as MYC were higher in lung cancer patients than in healthy volunteers, with statistically significant differences(P<0.005). The relative copy number of NFE2L2 and CYFRA21 were significantly positively correlated in lung cancer patients(P=0.031), and the copy number of MYC and KEAP1 were positively correlated(P=0.008).Conclusion:Gene copy number of MYC and NFE2L2 may be related to the mechanism of lung cancer development and progression.更多还原