【摘要】 目的:建立UPLC-MS/MS法同时测定排石合剂中京尼平苷酸、羟基红花黄色素A、绿原酸、苦杏仁苷、王不留行黄酮苷、甘草苷、毛蕊花糖苷、杯苋甾酮、丹皮酚、甘草酸铵10个特征成分的含量。方法:供试品经70%甲醇超声处理，Waters HSS T3 C₁₈（100 mm×2.1 mm, 1.7μm）色谱柱，以乙腈-0.1%甲酸为流动相进行梯度洗脱，流速0.3 mL·min^-1;电喷雾正负离子源同时监测，多反应监测（MRM）模式。结果:方法重复性、准确度良好，10个成分的平均回收率在99.0%～101.0%,RSD为0.090%～4.3%,在各自浓度范围内线性关系良好，含量结果分别为31.129～34.256、95.456～98.741、126.85～129.43、238.62～240.90、45.620～47.520、26.124～28.412、13.852～15.030、16.632～17.854、800.41～805.56、78.485～80.963μg·mL^-1。结论:本研究建立的方法快速简便，选择性好，准确可靠，为排石合剂的质量控制提供科学依据。更多还原

【Abstract】 Objective:To establish simultaneous determination method of 10 representative components in Paishi mixture, including geniposidic acid, hydroxyl-safflor yellow A, chlorogenic acid, amygdalin, vaccarin, liquiritin, mulberry glycoside, cyclamenone, paeonol and ammonium glycyrrhizinate. Methods: The sample was ultrasonicated with 70% methanol. The chromatographic separation was performed on a Waters HSS T3 C₁₈（100 mm×2.1 mm, 1.7 μm）column with acetonitrile-0.1% formic acid solution as mobile phase with gradient elution. The flow rate was 0.3 mL·min^-1. The analytes were detected by tandem mass spectrometry under the positive and negative ion modes with the ESI source and multiple reaction monitoring（MRM） mode. Results: The repeatability and accuracy of the method were good, and the average recoveries were 99.0%-101.0% with RSDs of0.090%-4.3%. The sample determination results showed that there were differences in the consistency between batches of preparations. The contents of 10 components were 31.129-34.256, 95.456-98.741, 126.85-129.43, 238.62-240.90, 45.620-47.520, 26.124-28.412, 13.852-15.030, 16.632-17.854, 800.41-805.56, 78.485-80.963 μg·mL^-1. Conclusion: The developed method is simple, rapid and accurate, which provides a more comprehensive scientific basis for the quality evaluation of Kechuan mixture.更多还原