【摘要】 目的 探索单种益生菌及两种益生菌联合对幽门螺杆菌(Helicobacter pylori,H.pylori)的抗菌效果，寻找最佳的抑菌组合。方法 用枯草芽孢杆菌、弯曲乳酸杆菌、嗜酸乳杆菌、粪肠球菌、长双歧杆菌等益生菌单独或两两联合与H.pylori共培养，测量益生菌对H.pylori的抑菌圈直径，并探索不同比例益生菌联合对H.pylori标准菌株和耐药菌株的抗菌效果。结果 单种的益生菌对H.pylori的抑制，枯草芽孢杆菌的抑制效果较好。与单种益生菌相比，枯草芽孢杆菌+嗜酸乳杆菌组合对耐药菌株的抑菌圈直径明显增大，差异有统计学意义(P<0.05);益生菌不同比例混合，枯草芽孢杆菌∶嗜酸乳杆菌=1∶1比枯草芽孢杆菌∶嗜酸乳杆菌=1∶10效果好(P<0.05),但枯草芽孢杆菌与嗜酸乳杆比例为20∶1、10∶1、1∶1三者之间差异无统计学意义(P>0.05)。结论 益生菌单用时，枯草芽孢杆菌对敏感或耐药H.pylori均具有较好抑制作用，联合益生菌的抑菌效果优于单独使用。联合应用枯草芽孢杆菌+嗜酸乳杆菌时，其按20∶1、10∶1、1∶1比例比1∶10时对H.pylori抗菌效果更佳，尤其是对临床耐药菌株。更多还原

【Abstract】 Objective To explore the antibacterial effects of single probiotics and the combination of two probiotics on Helicobacter pylori(H.pylori), and to find the best antibacterial combination. Methods Bacillus subtilis, Lactobacillus campylobacter, Lactobacillus acidophilus, Enterococcus faecalis, Bifidobacterium longum, and other probiotics were cultured alone or in combination with H. pylori. The diameter of bacteriostatic zone of probiotics against H. pylori was measured, and the antibacterial effects were explored in different proportion of probiotics on standard and drug-resistant strains of H. pylori. Results In terms of inhibiting H. pylori with single probiotics, Bacillus subtilis had the better effect. The diameter of antibacterial zone of the combination of Bacillus subtilis and Lactobacillus acidophilus on drug-resistant strains significantly increased, compared with that of single probiotics, and the difference was statistically significant(P<0.05). When probiotics were mixed at different ratios, the effect of Bacillus subtilis∶ Lactobacillus acidophilus = 1∶1 was better than that of Bacillus subtilis∶ Lactobacillus acidophilus=1∶10(P<0.05), but there was no statistical significance among the ratios(20∶1, 10∶1, 1∶1) of Bacillus subtilis to Lactobacillus acidophilus(P>0.05). Conclusion When probiotics are used alone, Bacillus subtilis has the better inhibitory effect on sensitive and drug-resistant H. pylori.The antibacterial effect of the combination of probiotics is better than that of single probiotics. The combination with Bacillus subtilis and Lactobacillus acidophilus mixed at the ratio of 20∶1, 10∶1 and 1∶1 has the better antibacterial effect on H. pylori than that mixed at the ratio of 1∶10, especially on clinical drug-resistant strains.更多还原