【摘要】 目的 基于动物模型系统评价益母草的毒性作用。方法 计算机检索中国知网、万方、维普、PubMed和Web of Science数据库，收集益母草毒性的动物实验研究，检索时间从数据库建立至2022年7月9日。2名研究人员分别对文献进行筛选和提取，采用SYRCLE风险偏倚评估工具评价纳入研究的偏倚风险，使用RevMan5.4软件进行meta分析。通过对给药时间、给药剂量及益母草提取方式进行亚组分析，以探索异质性来源，并根据敏感性分析来检验meta分析结果的稳定性。结果 共纳入18篇文献。meta分析显示：益母草超剂量使用能使干预组动物血中肝功能指标[谷丙转氨酶(ALT)、谷草转氨酶(AST)]、肾功能指标[肌酐(Cr)、尿素氮(BUN)]水平均高于对照组，差异均有统计学意义[SMD=1.01、1.05、1.72、1.46,95%CI(0.54,1.48)、(0.59,1.52)、(1.06,2.38)、(0.89,2.03),P<0.001]。亚组分析结果显示，给药时间对ALT、Cr和BUN的异质性水平影响不大，但可能是AST异质性的潜在来源；给药剂量对ALT、AST、Cr、和BUN的异质性无显著影响；益母草提取方式对Cr的异质性水平影响不大，可能会影响ALT、AST和BUN的异质性水平。结论 动物实验证据表明，益母草超剂量使用有造成肝肾功能损伤的风险。受研究数量和质量的限制，上述结论尚需更多的高质量研究予以验证。更多还原

【Abstract】 Objective To systematically evaluate the toxic effects of Leonurus heterophyllus based on animal models.Methods CNKI,Wanfang, VIP,PubMed and Web of Science databases were searched by computer to collect animal experimental studies on the toxicity of Leonurus heterophyllus.The search time was from the establishment of the database to July 9,2022.Two researchers screened and extracted the literature respectively.The SYRCLE risk bias assessment tool was used to evaluate the risk of bias of the included studies, and RevMan5.4 software was used for meta-analysis.Subgroup analysis was performed on the time of administration, the dose of administration and the extraction method of Leonurus heterophyllus to explore the sources of heterogeneity, and the stability of the meta-analysis results was tested according to the sensitivity analysis.Results A total of 18 articles were included.Meta-analysis showed that the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),creatinine(Cr) and urea nitrogen(BUN) in the intervention group were higher than those in the control group, and the differences were statistically significant[SMD= 1.01,1.05,1.72,1.46,95%CI(0.54,1.48),(0.59,1.52),(1.06,2.38),(0.89,2.03),P<0.001].The results of subgroup analysis showed that administration time had little effect on the heterogeneity levels of ALT,Cr, and BUN,but it may be a potential source of AST heterogeneity.The dosage has no significant effect on the heterogeneity of ALT,AST,Cr, and BUN.The extraction method of leonurus heterophyllus has little effect on the heterogeneity level of Cr, and may affect the heterogeneity level of ALT,AST,and BUN.Conclusion The current animal experimental evidence shows that the excessive use of Leonurus heterophyllus has the risk of causing liver and kidney function damage.Due to the limitation of the quantity and quality of the current research, the above conclusions need to be verified by more high-quality studies.更多还原