【摘要】 荧光探针基于荧光染料功能化修饰,实现对生物大分子、活性氧物种、活性硫物种、金属离子等物质的荧光检测。其非破坏性标记和检测属性使得他们在生命科学领域研究中备受青睐。近年来,随着染料精细化工、有机合成化学和荧光成像技术的共同创新,荧光成像在活体原位标记中不断取得新突破,有力地推动了活体荧光成像技术从基础研究向临床前应用迈进。本文中,我们将从荧光探针的基本概念出发,结合我们团队和其他同行的代表性研究成果,讨论荧光探针与活体荧光成像信噪比的相关性,展望其在促进生命科学领域分子水平认知的应用前景。更多还原

【Abstract】 Fluorescent probes can detect biological macromolecules, active oxygen species, active sulfur species, metal ions, etc. via fluorescence signal changes of the functional modification of fluorescent dyes. Their non-destructive labeling and detecting properties are favored by life science researchers. In recent years, with the joint innovation of dye chemical industry, organic synthetic chemistry and fluorescence imaging technology, fluorescence imaging has continuously made new breakthroughs in in situ labeling in vivo, which has effectively promoted the development of fluorescence imaging technology from basic research to pre-clinical application.This paper starts from the basic concept of fluorescent probe, combined with the representative research results of our team and others. It discusses the correlation between fluorescent probes and the signal-to-noise ratio of fluorescence imaging in vivo, and the application prospects of promoting molecular level cognition in life science.更多还原