【摘要】 目的 探讨17β-雌二醇和维生素K₂对人脐带间充质干细胞（human umbilical cord mesenchymal stem cells,h UC-MSCs）成骨分化的影响。方法 选取2019年11月内蒙古妇幼保健院的1份脐带标本，分离培养出h UC-MSCs。选择10^-10 mol/L17β-雌二醇（17β-estradiol,E₂）、10^-7 mol/L维生素K₂（vitamin K₂,VK₂），将细胞分为4组，分别为E₂组、VK₂组、E₂+VK₂组、对照组。实时荧光定量聚合酶链式反应检测目的基因骨基因Runt相关转录因子2（recombinant runt related transcription factor 2,RUNX2）、Ⅰ型胶原（type I collagen,COL1）、碱性磷酸酶（alkaline phosphatase,ALP）的表达。结果 成功提取出h UC-MSCs，通过茜素红、油红O、甲苯胺蓝染色证实h UC-MSCs具有多向分化的能力。结果显示与对照组相比，E₂或VK₂促进COL1（0.853、0.847）、RUNX2（0.830、0.757）、ALP（0.127、0.137）的表达，且联合组（COL1:1.003、RUNX2:1.170、ALP:0.163）作用更明显，差异有统计学意义（t=24.022、56.541、18.500;15.119、48.833、34.617;25.000、38.000、41.000,P<0.05）。结论 E₂和VK₂能够促进h UC-MSCs成骨分化，且联合作用效果更好。本研究为治疗骨损伤性疾病提供新的思路，同时为临床应用提供理论基础。更多还原

【Abstract】 Objective To investigate the effects of 17β-estradiol and vitamin K₂ on osteogenic differentiation of human umbilical cord mesenchymal stem cells （h UC-MSCs）.Methods A sample of umbilical cord was taken from Inner Mongolia Maternal and Child Health Care Hospital in November 2019,and h UC-MSCs were isolated and cultured.Select 10^-10 mol/L 17 β-estradiol （E₂）,10^-7 mol/L vitamin K₂（VK₂）,the cells were divided into four experimental groups,adding E₂ group,VK₂ group,E₂+VK₂ group,control group.The expressions of target genes recombinant runt related transcription factor 2,RUNX2,type I collagen,COL1 and alkaline phosphatase,ALP were detected by real-time polymerase chain reaction.Results HUC-MSCs were successfully extracted,and the multi-direction differentiation ability of h UC-MSCs was confirmed by alizarin red,oil red O and toluidine blue staining.The results showed that compared with the control group,E₂ or VK₂ promoted the expression of COL1（0.853,0.847）,RUNX2（0.830,0.757） and ALP（0.127,0.137）.The effect of the combined group （COL1:1.003,RUNX2:1.170,ALP:0.163） was more obvious,and the,and the difference was statistically significant （t=24.022,56.541,18.500;15.119,48.833,34.617;25.000,38.000,41.000,P<0.05）.Conclusion E₂ and VK₂ can promote the osteogenic differentiation of h UC-MSCs,and the combined effect is better.This study provides a new idea for the treatment of bone injury diseases,and provides a theoretical basis for clinical application.更多还原