【摘要】 叶片是植物进行光合作用等代谢过程的重要场所，所以对其形态建成及发育过程的研究非常重要. AS蛋白能够参与叶片极性建立的调控，但关于茶树AS蛋白的研究还较少.本研究从“福鼎大白茶”茶树中克隆获得了2个AS蛋白基因：CsAS1和CsAS2,编码蛋白长度分别为344 aa和229 aa,理论等电点分别为10.04和7.36,分别分布在第4和10号染色体上，均定位于细胞核；系统进化树和保守结构域分析表明，CsAS1和CsAS2蛋白间氨基酸序列和空间结构高度保守，且分别与葡萄和水稻的AS蛋白亲缘最近；CsAS1和CsAS2在茶树中的表达模式分析显示，CsAS1基因在茶树芽中的表达量最高，CsAS2基因在茶树根中的表达量最高，且CsAS1和CsAS2基因在茶树一芽二叶中的表达量均受到外源IAA和GA₃的诱导表达；启动子元件和转录组数据分析结果显示，CsAS1和CsAS2基因启动子序列中均含有多个非生物逆境胁迫响应的元件，且受到不同逆境胁迫的抑制或诱导表达.综上所述，CsAS1和CsAS2基因可参与茶树叶片的形态建成及非生物逆境胁迫的响应过程，可为进一步研究CsAS1和CsAS2基因在茶树叶片极性建立及叶片发育过程中的功能提供理论参考.更多还原

【Abstract】 Leaf is important place for photosynthesis and other metabolic processes in plants, and research of its morphogenesis and development processes are very crucial. AS protein can participate in the leaf polarity establishment. However, the studies on AS protein in tea plant were relatively few. In this study, two AS protein genes, CsAS1 and CsAS2 were identified and cloned from ‘Fuding Dabaicha’ plant, encoding 344 aa and 229 aa, respectively. The pI of CsAS1 and CsAS2 were 10.04 and 7.36, respectively. CsAS1 and CsAS2 were distributed on chromosome 4 and 10, respectively, and both CsAS1 and CsAS2 located in the nucleus. Phylogenetic analysis and conserved domain analysis showed that CsAS1 and CsAS2 were highly conserved in amino acid sequence and space structures of the proteins, and had the closest protein affinity to grape and rice, respectively. The results of expression analysis showed that the highest level of CsAS1 expression was in the bud, and the highest level of CsAS2 expression was in the root. Moreover, the expressions of CsAS1 and CsAS2 in the shoot tip with one bud and two leaves were induced by exogenous IAA and GA₃ treatment. The analysis of promoter and transcriptome data showed that the both promoter sequences contained multiple abiotic stress response elements, and expressions of CsAS1 and CsAS2 were inhibited or induced by different stresses. In summary, CsAS1 and CsAS2 could participate in the morphogenesis of leaves and the process of abiotic stress response in tea plants, which could provide the reference for further research on the function of CsAS1 and CsAS2 in the polarity of tea leaves and leaf development.更多还原