【摘要】 目的：研究胃癌耐药相关hsa-miR-194-5p的靶基因，并分析其参与的生物学过程和信号转导通路，为胃癌耐药机制的表观遗传学研究提供前期基础.方法：通过NCBI数据库检索hsa-miR-194-5p的基本信息；使用预测数据库预测hsa-miR-194-5p的靶基因；使用DAVID数据库对靶基因集合进行功能富集分析(GO)和信号转导通路富集分析(KEGG).结果：成熟的miR-194-5p序列多个物种之间具有高度保守性.预测靶基因共325个，参与的生物学过程主要有Golgi组织和细胞对DNA损伤刺激的反应(P<0.01),分子功能包括泛素蛋白连接酶结合和泛素介导的蛋白水解(P<0.01),细胞组分富集在细胞核和细胞表面，调控包括TGF-β和WNT等多条信号转导通路(P<0.01).结论：miR-194-5p的靶基因SMURF1、 RAC1、MAPK1与胃癌细胞的增殖密切相关.更多还原

【Abstract】 Objective: To further study the target genes of hsa-miR-194-5p related to drug resistance in the gastric cancer and analyze the biological processes and signal transduction pathways involved in theses processes, so as to provide a preliminary basis for the epigenetic study of drug resistance mechanism in the gastric cancer.Methods: The basic information of hsa-miR-194-5p was retrieved from NCBI database.The target genes of hsa-miR-194-5p were predicted using the prediction database.Functional enrichment analysis(GO) and signal transduction pathway enrichment analysis(KEGG) were performed on the target gene set using the DAVID database.Results: The mature miR-194-5p sequence is highly conserved among multiple species.A total of 325 target genes were predicted.The biological processes involved were mainly the response of Golgi tissues and cells to DNA damage stimulation(P<0.01).Molecular functions included ubiquitin ligase binding and ubiquitin-mediated proteolysis(P<0.01).Cell components were enriched in the nucleus and cell surface, and regulated multiple signal transduction pathways including TGF-β and WNT(P<0.01).Conclusion: The target genes SMURF1,RAC1 and MAPK1 of miR-194-5p are closely related to the proliferation of gastric cancer cells.更多还原