【摘要】 旨在探究鼠伤寒沙门菌及沙门菌血清型4,[5],12:i:-基因组学特征。采用Illumina NovaSeq 6000平台对91株河南省猪产业链中鼠伤寒沙门菌及沙门菌血清型4,[5],12:i:-分离菌进行全基因组测序，通过生物信息学分析，进行其血清型的预测、多位点序列的分型以及质粒类型、耐药基因和毒力基因分析。结果表明，在河南省猪产业链中沙门菌4,[5],12:i:-的流行率(1.58%, 50/3 173)已经超过鼠伤寒沙门菌的流行率(1.29%, 41/3 173);91株沙门菌的序列型分为ST34(71.42%,65/91)和ST19(28.57%,26/91);鼠伤寒沙门菌/ST34菌株和4,[5],12:i:-/ST34菌株都是IncHI2A＿1、IncHI2＿1和Col440I＿1质粒的偏好宿主；沙门菌血清型4,[5],12:i:-携带率较高的耐药基因是blaTEM-1B＿1(72%)、tet(B)＿2(90%)、sul2＿3(66%)、blaOXA-1＿1(34%)等；相比鼠伤寒沙门菌菌株，沙门菌血清型4,[5],12:i:-菌株中质粒的携带率更高，且携带更多种类的耐药基因和毒力基因。本研究首次证实，河南省猪产业链中沙门菌血清型4,[5],12:i:-的流行已经超过鼠伤寒沙门菌，在一定程度上解释了该血清型在河南省病人中主要流行的原因。更多还原

【Abstract】 Whole genomes of 91 Salmonella isolates from pig-borne food chain of Henan province were sequenced by Illumina NovaSeq 6000 platform for the understanding of genomic characteristics of Salmonella typhimurium and Salmonella serotype 4,[5],12:i:-. Serotype prediction, multi-locus sequence typing, plasmid types, virulence genes and drug resistance genes were analyzed by bioinformatics. The results showed that the prevalence of Salmonella 4,[5],12:i:-(1.58%, 50/3 173) was higher than that of Salmonella typhimurium(1.29%, 41/3 173) in pig-borne food chain of Henan Province. Sequence types(STs) of 91 Salmonella isolates included ST34(71.42%, 65/91) and ST19(28.57%, 26/91). Typhimurium/ST34 strains and 4,[5],12:i:-/ST34 strains were the preferred hosts of some plasmids including IncHI2A＿1, IncHI2＿1 and Col440I＿1. Resistant genes highly carried by Salmonella serotypes 4,[5],12:i:-were blaTEM-1B＿1(72%), tet(B)＿2(90%), sul2＿3(66%), blaOXA-1＿1(34%), etc. Compared with Salmonella typhimurium isolates, Salmonella serotype 4,[5],12:i:-isolates had a higher plasmid-carrying rate, carrying a greater variety of resistance genes and virulence genes. This study confirmed for the first time that the prevalence of Salmonella serotype 4,[5],12:i:-has exceeded that of Salmonella typhimurium in pig-borne food chain of Henan Province. To a certain extent, this may explain the predominant prevalence of the Salmonella serotype among patients in the area.更多还原