【摘要】 目的 探讨通过微小RNA-519d-3p(miR-519d-3p)调控Toll样受体4/核因子-κB(TLR4/NF-κB)信号通路对前列腺癌细胞的机制研究。方法 于2021年6月购买前列腺癌细胞株PC3,培养至对数时期，随机分为对照组、下调miR-519d-3p组、上调miR-519d-3p组。采用聚合酶链反应检测miR-519d-3p表达情况，对比干预24h、48h、72h细胞增殖、细胞迁移、侵袭率；检测细胞TLR4/NF-κB信号通路蛋白表达状况。结果 与对照组相比，下调miR-519d-3p组细胞增殖率、细胞侵袭数、迁移数、miR-519d-3p表达量、B淋巴细胞瘤-2基因/Bcl-2相关X基因(Bcl-2/Bax)、TLR4/β-actin、NF-κB/β-actin蛋白表达量上升，细胞凋亡率下降(P<0.05);与下调miR-519d-3p组相比，上调miR-519d-3p组细胞增殖率、细胞侵袭数、迁移数、miR-519d-3p表达量、Bcl-2/Bax、TLR4/β-actin、NF-κB/β-actin蛋白表达量降低，细胞凋亡率升高(P<0.05)。结论 上调miR-519d-3p可抑制前列腺癌细胞的增殖、迁移、侵袭，其作用机制可能与TLR4/NF-κB信号通路相关。更多还原

【Abstract】 Objective To investigate the mechanism of miR-519d-3p(miR-519d-3p) on prostate cancer cells by regulating toll-like receptor 4/nuclear factor-κB(TLR4/NF-κB) signaling pathway. Methods Prostate cancer cell line PC3 was purchased in June 2021 and cultured to the logarithmic stage. The patients were randomly divided into control group, down-regulated miR-519d-3p group and up-regulated miR-519d-3p group. The expression of miR-519d-3p was detected by polymerase chain reaction, and the cell proliferation, migration and invasion rates were compared after 24h, 48h and 72h. The protein expression of TLR4/NF-κB signaling pathway was detected. Results Compared with the control group, the cell proliferation rate, cell invasion number, migration number, miR-519d-3p expression, B lymphoblastoma-2 gene/Bcl-2 Bax gene(Bcl-2/Bax), TLR4/β-actin, NF-κB/β-actin protein expression increased in down-regulated miR-519d-3p group, cell apoptosis rate decreased(P<0.05). Compared with the down-regulated miR-519d-3p group, the cell proliferation rate, cell invasion number, migration number, miR-519d-3p expression, Bcl-2/Bax, TLR4/β-actin, NF-κB/β-actin protein expression decreased in the up-regulated miR-519d-3p group, the apoptosis rate was increased(P<0.05). Conclusions The up-regulation of miR-519d-3p can inhibit the proliferation, migration and invasion of prostate cancer cells, and its mechanism may be related to TLR4/NF-κB signaling pathway更多还原