【摘要】 目的 分析Collinsella aerofaciens(C.aerofaciens,分组中简写为CA)对高脂饮食小鼠肠道菌群及其代谢短链脂肪酸水平的影响，初步探讨肠道菌群对血脂代谢的作用机制。方法 将40只6～8周龄的C57BL/6雄性小鼠随机分为：正常饮食组(NCD组)、正常饮食+CA菌组(NCD+CA组)、高脂饮食组(HFD组)、高脂饮食+CA菌组(HFD+CA组),每组10只。NCD和HFD组小鼠给予生理盐水100μL/只/天，其余各组给予10~9 CFU/mL C.aerofaciens菌液100μL/只/天，干预12 w后，收集小鼠血清，通过试剂盒检测血脂水平；收集各组小鼠粪便，采用高通量测序技术和气相色谱法分别检测肠道菌群结构变化和短链脂肪酸水平。结果 在第12周，与NCD组相比，HFD组小鼠总胆固醇(TC)和甘油三酯(TG)水平明显升高(P<0.05);与HFD组相比，HFD+CA组小鼠TC水平明显升高(P<0.05)。分析4、8和12 w各组小鼠短链脂肪酸水平，与NCD组相比，NCD+CA组(P<0.001)、HFD组(P<0.01)、HFD+CA组(P<0.01)丙酸水平显著下降，与HFD组相比，HFD+CA组乙酸、丙酸、丁酸水平无明显差异(P>0.05)。相关性分析表明，丙酸与Bacteroides、Odoribacter等呈正相关，与Mucispirillum、M.schaedleri呈负相关。结论 C.aerofaciens诱发小鼠血脂代谢紊乱，可能与其调节肠道菌群结构，影响菌群代谢短链脂肪酸水平有关，但其机制需进一步研究。更多还原

【Abstract】 Objective To analyze the effect of Collinsella aerofaciens(C. aerofaciens, abbreviated as CA in grouping) on gut microbiota and its metabolized short-chain fatty acids in mice fed a high-fat diet, and to preliminarily investigate the relationship between the gut microbiota and lipid metabolism. Methods 40 C57BL/6 male mice aged 6-8 weeks were randomly divided into normal diet group(NCD), normal diet+CA group(NCD+CA), high fat diet group(HFD) and high fat diet+CA group(HFD+CA), with 10 mice in each group. Mice in the NCD and HFD groups were given 100 μL/mouse/day of normal saline, and the other groups were given 100 μL/mouse/day of 10~9 CFU/mL CA bacterial solution, and after 12 weeks of intervention, mouse sera were collected to detect blood lipid levels by kits; mouse feces were collected from the each group to detect intestinal microbiota structural changes and short-chain fatty acid levels with using gas chromatography, respectively. Results At week 12, compared with NCD,total cholesterol(TC) and triglyceride(TG) levels were significantly increased in HFD mice(P<0.05); compared with HFD,TC levels were significantly increased in HFD+CA mice(P<0.05). Analysis of short-chain fatty acid levels in each group of mice at 4, 8 and 12 weeks showed that propionic acid levels were significantly decreased in the NCD+CA(P<0.001), HFD(P<0.01), and HFD+CA(P<0.01) groups compared with the NCD group, and acetic acid, propionic acid, and butyric acid levels were not significantly different in the HFD+CA group compared with the HFD group(P>0.05). Correlation analysis showed that propionic acid was positively correlated with Bacteroides and Odoribacter, and negatively correlated with Mucispirillum and M. schaedleri. Conclusion C. aerofaciens can induce lipid metabolism disorders in mice, which may be related to its regulation of gut microbiota structure and the effects on microbiota metabolic short-chain fatty acid levels, but the mechanism needs further study.更多还原