【摘要】 【目的】比较不同来源葡萄果实中叶酸积累的差异，并利用分子标记技术对高叶酸种质资源进行评价和筛选。【方法】采用ELISA技术检测22份葡萄种质资源果实叶酸含量；利用ISSR分子标记对22份葡萄种质资源进行基因组多态性分析和聚类分析；分析检测出叶酸含量与聚类结果。【结果】22份葡萄种质资源果实叶酸含量的范围在1.93～46.95 ng/g,在遗传相似系数为0.621处，22份葡萄材料明显分为2大类群。第1大类叶酸含量平均为35.79 ng/g;其余为第2大类，叶酸含量平均为15.71 ng/g。在遗传相似系数为0.622处，21份葡萄材料又明显分为2大类群。第1大类叶酸含量平均为16.53 ng/g;第2大类叶酸含量平均为12.24 ng/g。欧亚种的叶酸含量较欧美杂种稍高3.998 7 ng/g;从30条引物中筛选出9条扩增稳定且多态性丰富的引物用于葡萄的ISSR扩增。共扩增出78条条带，其中多态性条带70条，多态性百分率为89.7%。22份葡萄种质资源的遗传相似系数为0.474 4～0.846 2,平均遗传相似系数为0.654 5。在遗传相似系数为0.621处，22份葡萄材料明显分为2大类群。第1大类包含1个欧亚种(红巴拉多),其余为第2大类。在遗传相似系数为0.622处，21份葡萄材料又明显分为2大类群。第1大类包含11个欧亚种(炎兴无核、灰比诺、瑞都翠霞、京蜜、黑奇无核、无核白、1124、京翠、红宝石、无核紫、达米娜),6个欧美杂种(杂1、夏黑、蜜红、红标无核、洛浦早生、宇选1号)。第2大类包含4个欧亚种(美人指、粉红亚都蜜、木纳格、里扎马特)。【结论】群体1的叶酸含量比群体2的叶酸含量高，欧亚种的叶酸含量较欧美杂种稍高。更多还原

【Abstract】 【Objective】 To compare the differences of folic acid accumulation in grape fruits from different sources and to evaluate and screen high folic acid germplasm resources by molecular marker technology.【Methods】 The folic acid content of 22 grape germplasm resources was detected by ELISA; The genomic polymorphism and cluster analysis of 22 grape germplasm resources were carried out by ISSR molecular markers; The detected folic acid content was analyzed with the clustering results.【Results】 The fruit folic acid content of 22 grape germplasm resources ranged from 1.93 to 46.95 ng/g.Compared with the clustering results, the 22 grape germplasm resources were obviously divided into two groups at the genetic similarity coefficient of 0.621.The average folic acid content of the first category was 35.79 ng/g; The rest belonged to the second category, with an average folate content of 15.71 ng/g.At the genetic similarity coefficient of 0.622, the 21 grape materials were obviously divided into 2 groups.The average folic acid content of the first category was 16.53 ng/g and the average folic acid content of the second category was 12.24 ng/g.At the same time, the analysis of variance showed that the folic acid content of Eurasian species was slightly higher than that of European and American hybrids by 3.998,7 ng/g; Through ISSR molecular marker technology, 9 primers with stable amplification and abundant polymorphism were selected from 30 primers for ISSR amplification of grape.A total of 78 bands were amplified, including 70 polymorphic bands, with a polymorphic percentage of 89.7%.According to the ISSR amplification results, the Jaccard similarity coefficient was analyzed by NTSYSpc2.10e software, and the cluster analysis was carried out by unweighted paired arithmetic mean(UPGMA) to establish the genetic relationship map.The genetic similarity coefficient of 22 grape germplasm resources was between 0.474,4-0.846,2, and the average genetic similarity coefficient was 0.654,5.At the genetic similarity coefficient of 0.621, 22 grape materials were obviously divided into 2 groups.The first category included one Eurasian species(red balado), and the rest was the second category.At the genetic similarity coefficient of 0.622, the 21 grape materials were obviously divided into 2 groups.The first category included 11 Eurasian species(Yanxing seedless, Pinot Grigio, Ruidu Cuixia, Jingmi, Heiqi Seedless, Seedless White, 1124, Jingcui, Ruby, Seedless Purple, Damina), and 6 European and American hybrids(ZA 1, Xiahei, Mihong, Hongbiao Seedless, Luopu Zaosheng and Yuxuan 1).The second category included 4 Eurasian species(Beauty finger, Pink Yadumi, Munag and Rizamat).【Conclusion】 Through comparing and analyzing the fruit folic acid content and clustering results of different grape germplasm resources, it can be seen that the folic acid content of population 1 is higher than that of population 2.The folic acid content of Eurasian species was slightly higher than that of European and American hybrids.更多还原