【摘要】 目的 探讨鞣花酸在特发性膜性肾病治疗中可能的意义及作用机制。方法 将SD大鼠(SPF级、8周龄)随机分为实验组、模型组和对照组，实验组和模型组各8只大鼠，对照组6只大鼠。实验组和模型组通过注射阳离子化牛血清白蛋白构建特发性膜性肾病大鼠模型。造模成功后，实验组按200 mg/kg体重腹腔注入鞣花酸，注射频率为1次/日、持续4周，模型组注入等体积生理盐水。四周后各组大鼠行异氟烷麻醉后处死，收集各组血液、尿液和肾脏标本进行进一步的生化、病理及分子生物学分析。结果 采用鞣花酸干预的特发性膜性肾病大鼠的肾功能指标(血肌酐、尿素氮、血清白蛋白、24 h尿蛋白和血胆固醇)和肾小管性尿蛋白各组分(α1-MG、β2-MG、RPB、NAG和NGAL)与模型组相比均显著好转(P<0.05),且病理损伤明显减轻；实验组还原谷胱甘肽(GSH)、过氧化酶(CAT)较对照组及模型组均显著上调(P<0.05)。与模型组相比，实验组大鼠Mst1 mRNA表达下降(P<0.05),Nrf2 mRNA表达增加(P<0.05)。结论 鞣花酸可能通过Mst1-Nrf2通路减轻肾组织的氧化应激从而缓解C-BSA诱导的膜性肾病大鼠发生肾脏病理损伤。更多还原

【Abstract】 Objective To explore the possible significance and mechanism of ellagic acid in the treatment of IMN, and look for potential options for the treatment of IMN.Methods SD rats(SPF, 8 weeks) were randomly divided into experimental group, model group and control group, with 8 rats in the experimental group and 8 rats in the model group and 6 rats in the control group. The experimental group and model group were injected with cationic bovine serum albumin(BSA) to construct a rat model of idiopathic membranous nephropathy. After successful modeling, the experimental group was intraperitoneally injected ellagic acid at the rate of 200mg/kg body weight once a day for 4 weeks, and the model group was injected with normal saline of the same volume. Four weeks later, the rats in each group were anesthetized with isoflurane and killed. Blood, urine and kidney samples were collected for further biochemical, pathological and molecular biological analysis.Results The renal function indexes(serum creatinine, urea nitrogen, serum albumin, 24-hour urine protein and blood cholesterol) and the components of renal tubular urine protein(α1-MG, β2-MG, RPB, NAG and NGAL) of rats with idiopathic membranous nephropathy treated with ellagic acid were significantly improved, and the P values were all less than 0.05 compared with model group. Compared with model group, Mst1 mRNA expression of experimental rats decreased(P<0.05), while Nrf2 mRNA expression increased(P<0.05). Conclusion Ellagic acid may alleviate oxidative stress in renal tissue through the MST1-NRF2 pathway and thus alleviate renal pathological damage in C-BSA induced IMN.更多还原