【摘要】 目的 探讨雨蛙素诱导的大鼠急性胰腺炎肠道菌群的影响，挖掘与其发生高度相关的肠道共生菌。方法 将雄性Sprague Dawley大鼠随机分为造模组(AP组)和对照组(Control组)。通过腹腔注射雨蛙素构建大鼠急性胰腺炎模型，收集血清标本检测血清淀粉酶、脂肪酶。收集胰腺组织，采用HE染色法观察胰腺组织病理变化。收集大鼠粪便，借助16S rRNA测序技术研究两组大鼠肠道微生物菌群的变化，并通过与大鼠生理指标表型进行关联分析进而挖掘肠道共生菌。结果 雨蛙素诱导的急性胰腺炎可显著升高大鼠血清淀粉酶和脂肪酶活力(P<0.01),降低胰腺干湿比(P<0.01),造成胰腺组织结构弥漫性破坏；16S rRNA测序结果显示，两组间肠道菌群存在差异，其中急性胰腺炎组肠道菌群多样性显著降低，属水平上以埃希氏-志贺氏菌属、拟杆菌属和肠球菌属为主要优势属。结论 雨蛙素诱导的大鼠急性胰腺炎引起肠道菌群的多样性、丰富度和均匀度降低及物种组成发生显著改变。更多还原

【Abstract】 Objective To explore the effect of cerulein on Gut microbiota in rats with acute pancreatitis, and to explore the intestinal symbiotic bacteria highly related to its occurrence. Methods Male Sprague Dawley rats were randomly divided into model group(AP group) and control group(Control group). The rat model of acute pancreatitis was constructed by intraperitoneal injection of caerulein, and serum samples were collected to detect serum amylase and lipase. Pancreatic tissue was collected and pathological changes of pancreatic tissue were observed by HE staining. The feces of rats were collected, and the changes of intestinal microflora in the two groups were studied by 16S rRNA sequencing technology. The intestinal symbiotic bacteria were explored by correlation analysis with the physiological indexes of rats. Results Acute pancreatitis induced by caerulein could significantly increase the activity of serum amylase and lipase(P<0.01), reduce the dry-wet ratio of the pancreas(P<0.01), cause diffuse destruction of pancreatic tissue structure. 16S rRNA sequencing showed that there were differences in intestinal microbiota between the two groups. The diversity of intestinal flora in the acute pancreatitis group was significantly reduced, and Escherichia-Shigella, Bacteroides and Enterococcus were the main dominant genera at the genus level. Conclusion Acute pancreatitis induced by cerulein in rats can reduce the diversity, richness, and evenness of intestinal flora, and significantly change the species composition.更多还原