【摘要】 目的 通过采用小干扰RNA(siRNA)靶向作用人肝癌细胞Bel-7402中的水通道蛋白5(AQP5)基因，观察其对Bel-7402的增殖及凋亡的作用。方法 体外孵育Bel-7402,采用阴性对照siRNA(siRNA-NC)、siRNA-AQP5#1、siRNA-AQP5#2转染细胞。实验设置细胞为对照组、siRNA-NC组、sliRNA-AQP5#1组、siRNA-AQP5#2组。噻唑蓝染色检测细胞的增殖能力；流式细胞技术测定细胞的凋亡情况；蛋白免疫印迹技术和Real time-PCR测量细胞中AQP5蛋白和mRNA的含量。结果 siRNA-NC组与对照组相比，细胞的增殖、凋亡及AQP5蛋白和mRNA的含量无明显差异(P>0.05)。与对照组相比，siRNA-AQP5#1组、siRNA-AQP5#2组的细胞的增殖力降低、凋亡率升高、AQP5蛋白和mRNA的含量均降低(均P<0.05)。结论 siRNA靶向作用人肝癌细胞AQP5基因通过降低其增殖和升高其凋亡率达到抑制癌细胞的生长作用。更多还原

【Abstract】 Objective The small interfering RNA(siRNA) targeting the aquaporin 5(AQP5) gene in human hepatoma cell Bel-7402 was used, we observe its effects on the proliferation and apoptosis of Bel-7402. Methods Bel-7402 was incubated in vitro and transfected with negative control siRNA(siRNA-NC),siRNA-AQP5#1 and siRNA-AQP5#2. The cells were set as control group, siRNA NC group, siRNA-AQP5#1 group and siRNA-AQP5#2 group. The proliferation ability of cells was detected by thiazole blue staining; The apoptosis of cells was measured by flow cytometry; The contents of AQP5 protein and mRNA in cells were measured by Western blot and real time PCR. Results The cell proliferation, apoptosis, AQP5 protein and mRNA had not obvious difference between siRNA-NC group and control group(P>0.05). Marched with the control group, the proliferation of cells decreased, the apoptosis rate increased, and the contents of AQP5 protein and mRNA decreased in siRNA-AQP5#1 group and siRNA-AQP5#2 group(all P<0.05). Conclusion siRNA targeting AQP5 gene can inhibit the growth of human hepatoma cells by reducing their proliferation and increasing their apoptosis rate.更多还原