【摘要】 背景：松弛素是人松弛素2的重组形式，作为机体内源性抗纤维化活性物质，松弛素治疗可能会缓解急性心力衰竭患者的症状并改善其预后，但尚未有研究松弛素在氧化应激方面对缺氧/复氧损伤小鼠心肌微血管内皮细胞(H5V)的作用。目的：探讨松弛素在小鼠H5V细胞缺氧/复氧损伤模型中的作用。方法：建立小鼠H5V细胞缺氧/复氧损伤模型，分别为缺氧3,6,12 h，复氧3 h，通过检测细胞活力和乳酸脱氢酶活性确定细胞缺氧/复氧的最佳时间。选用不同质量浓度的松弛素(0,60,100,140,180 ng/mL)作用于H5V细胞缺氧/复氧损伤模型，通过检测细胞活力和乳酸脱氢酶活性确定松弛素干预的最佳质量浓度。在此基础上，将H5V细胞分为3组：对照组、缺氧/复氧模型组、缺氧/复氧+180 ng/mL松弛素组，检测丙二醛、超氧化物歧化酶和活性氧水平。结果与结论：(1)与对照组比较，缺氧6 h/复氧3 h、缺氧12 h/复氧3 h的细胞活力均显著降低(P <0.05)，细胞上清中乳酸脱氢酶活性均显著增加(P <0.05)，选取缺氧6 h/复氧3 h进行后续实验；(2)与缺氧/复氧模型组比较，缺氧/复氧+松弛素180 ng/mL组细胞活力显著增加(P <0.05)，细胞上清中乳酸脱氢酶活性显著降低(P <0.05)，选取松弛素180 ng/mL进行后续干预实验；(3)与缺氧/复氧模型组比较，缺氧/复氧+180 ng/mL松弛素组活性氧、丙二醛水平显著降低(P <0.05)，超氧化物歧化酶水平显著增加(P <0.05)；(4)结果表明，H5V细胞经缺氧6 h/复氧3 h可建立H5V细胞损伤模型，经180 ng/mL松弛素干预后提高了细胞活性和细胞抗氧化应激能力。更多还原

【Abstract】 BACKGROUND: Serelaxin is a recombinant form of human relaxin 2. As endogenous anti-fibrosis active substances, serelaxin may relieve the symptoms and improve the prognosis of patients with acute heart failure. However, there is no report on the effect of serelaxin on hypoxia/reoxygenation injury of myocardial microvascular endothelial cells(H5V) in mice through regulating oxidative stress.OBJECTIVE: To investigate the role on serelaxin in a model of hypoxia/reoxygenation-induced mouse cardiac microvascular endothelial cells(H5V)injury.METHODS: To establish a mouse H5V hypoxia/reoxygenation injury model, cells were incubated under hypoxia for 3, 6, and 12 hours followed by 3 hours of reoxygenation. The optimal time of cellular hypoxia/reoxygenation was determined by cell viability and lactate dehydrogenase activity assay. Different concentrations of serelaxin(0, 60, 100, 140, 180 ng/mL) were selected to act in the H5V cellular hypoxia/reoxygenation injury model, and the optimal drug concentration for drug treatment of cellular hypoxia-reoxygenation injury was determined by the cell viability and lactate dehydrogenase activity assays.H5V cells were then divided into three groups: control, model(hypoxia/reoxygenation), and serelaxin(hypoxia/reoxygenation+180 ng/mL serelaxin) groups.Malondialdehyde, superoxide dismutase and reactive oxygen species levels were detected in each group.RESULTS AND CONCLUSION: Compared with the control group, cell proliferation was significantly reduced after hypoxia 6 hours/reoxygenation 3 hours and hypoxia 12 hours/reoxygenation 3 hours(P < 0.05), while the lactate dehydrogenase activity in cell supernatant was significantly increased(P < 0.05). Therefore,cells cultured under hypoxia 6 hours/reoxygenation 3 hours were selected for subsequent experiments. Cell viability was significantly increased in the serelaxin group compared with the model group(P < 0.05), while the lactate dehydrogenase activity in cell supernatant was significantly decreased(P < 0.05). Therefore,180 ng/mL serelaxin was selected for subsequent experiments. Compared with the model group, the serelaxin group showed a significant decrease in reactive oxygen species and malondialdehyde levels but a significant increase in the level of superoxide dismutase(all P < 0.05). To conclude, H5V cells subjected to hypoxia 6 hours/reoxygenation 3 hours could establish a model of H5V vascular endothelial cell injury, and 180ng/mL serelaxin could improve the cell viability and anti-oxidative stress ability.更多还原