【摘要】 目的:探讨Fms样酪氨酸激酶1(Flt-1)基因rs722503多态性与青海省汉族妊娠期高血压疾病(HDP)的相关性，并初步确定该基因在HDP组中的表达情况。方法:选择青海省汉族HDP患者143例(HDP组),正常汉族孕妇133例(对照组),以血液DNA为模板，采用限制性内切酶片段长度多态性聚合酶链反应(PCR-RFLP)检测患者rs722503分型并测序验证。实时荧光定量PCR(RT-PCR)法检测胎盘组织中Flt-1 mRNA表达。酶联免疫吸附实验(ELISA)检测血清可溶性Fms样酪氨酸激酶1(sFlt-1)水平。结果:HDP组和对照组rs722503 AA、AG、GG基因型频率分别为54.55%、41.26%、4.19%和40.60%、50.38%、9.02%,两组基因型分布差异有统计学意义(P<0.05),其中AA基因型(OR=1.756,95%CI=1.89～2.830,P<0.05)是导致HDP发生的危险因素。HDP组和对照组rs722503 A(OR=1.143,95%CI=1.024～1.275)和G(OR=0.726,95%CI=0.559～0.943)等位基因频率分布差异有统计学意义(P<0.05)。HDP组胎盘组织中Flt-1 mRNA相对表达量高于对照组(P<0.01)。HDP组血清sFlt-1水平[(7.04±1.94)ng/mL]高于对照组[(2.92±1.02)ng/mL](P<0.01)。结论:Flt-1基因rs722503与青海省汉族HDP相关，其中AA基因型可能是HDP的易感基因型，A等位基因可能是HDP的易感基因，G等位基因可能是HDP的保护基因。胎盘组织Flt-1 mRNA相对表达量及血清sFlt-1蛋白水平可能与青海省汉族HDP相关。更多还原

【Abstract】 Objective:To investigate the relationship between rs722503 polymorphism of fms-like tyrosine kinase-1(Flt-1) gene and hypertensive disorder of pregnancy(HDP) in Han nationality of Qinghai Province and to determine the expression of this gene in HDP group and control group preliminarily.Methods:A total of 143 Han nationality HDP subjects(HDP group) and 133 Han nationality normal pregnant subjects(control group) were selected from Qinghai province.The rs722503 typing in HDP group and control group was analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and verified by sequencing.The expression of Flt-1 mRNA in the placental tissues of the two groups were detected by real-time PCR.Serum soluble fms-like tyrosine kinase-1(sFlt-1) levels of the two groups were detected by enzyme-linked immunosorbent assay(ELISA).Results:The frequency of AA,AG,GG genotype of rs722503 in HDP group and control group were 54.55%,41.26%,4.19% and 40.60%,50.38%,9.02% respectively(P<0.05),there were significant differences between the two genotypes, AA genotype(OR=1.756,95%CI=1.89～2.830,P<0.05) was a risk factor for HDP.The frequency distribution of alleles A(OR=1.143,95%CI=1.024～1.275) and G(OR=0.726,95%CI=0.559～0.943) of rs722503 were significant different between HDP group and control group(P<0.05).The relative expression of Flt-1mRNA in placental tissue of HDP group was higher than that of control group(P<0.01).The serum sFlt-1 level in HDP group[(7.04 ± 1.94)ng/mL] was higher than that in control group[(2.92±1.02)ng/mL](P<0.01).Conclusion:The rs722503 polymorphism of Flt-1 gene is significantly correlated with HDP of Han nationality in Qinghai Province, the AA genotype may be the susceptibility genotype of HDP,the A allele may be the susceptibility gene of HDP,the G allele may be the protective gene of HDP.The relative expression of Flt-1mRNA in placental tissue and the level of serum sFlt-1 protein may be correlated with HDP of Han nationality in Qinghai Province.更多还原