【摘要】 该研究设计了一门以科学素养培养为目标的科学技能综合实验课程,该课程由科研讲座、基础实验和创新实验组成,科研讲座对科研思路、方法等进行介绍,帮助学生形成正确、严谨、完整的科学研究架构;基础实验以NLRP3炎性小体为研究对象,基于科研基本逻辑,依次利用生物信息学、分子克隆及细胞生物学、免疫学相关方法对NLRP3结构域的功能进行研究;创新实验以基础实验的结果和方法为基础,对NLRP3的功能和结构等进行进一步探究。该课程涵盖多学科重点实验技术,科研讲座、基础实验与创新实验有机融合,保证重点实验技术培养和相关理论知识的深化理解,同时也为学生提供了较大的创新空间,有利于学生树立良好的科研思维和习惯,为学生提供相对完整的科研训练。同时,该课程相关技术成熟、结果稳定、可行性高,值得推广。更多还原

【Abstract】 A comprehensive biotechnology experimental course aiming at the cultivation of scientific literacy was designed, which was composed of scientific research lectures, basic experiments and innovative experiments. Scientific research ideas and methods were introduced in lectures, which would help students form a correct,rigorous and complete scientific research framework; the basic experiment takes NLRP3 inflammasome as the research object. Based on the basic logic of scientific research, the structural domain and function of NLRP3 inflammasome are studied using bioinformatics, molecular cloning, cell biology and immunology methods; based on the results and methods of basic experiments, the innovation experiment further explored the function and structure of NLRP3. The project covers multi-disciplinary key experimental technologies. Scientific research lectures, basic experiments and innovative experiments are organically integrated to ensure the cultivation of key experimental technologies and the deepening understanding of relevant theoretical knowledge. At the same time, it provides a large space for innovation, which is conducive to students to establish good thought and habits for scientific research,and provides students with relatively complete scientific research training. At the same time, the project has mature technology, stable results and high feasibility, which is worth promoting.更多还原