【摘要】 应用电子扫描电镜、电子顺磁共振波谱仪对解淀粉芽孢杆菌以亚硒酸钠为硒盐转化的纳米硒进行表征。结果表明：解淀粉芽孢杆菌转化生成的纳米硒尺寸约300 nm,大小均一，结晶度高，且在分离提纯后的纳米硒上检测到活性氧和微弱的环境持久性自由基的信号。研究结果阐释了微生物产生纳米硒的物理化学性状，并对纳米硒产生过程中一类新型物质-环境持久性自由基的产生进行了评估，为全面理解微生物转化纳米硒提供了新视角。更多还原

【Abstract】 The sodium selenite was used as selenium salt, the nano-selenium(SeNPs) transformed by Bacillus amyloliquefaciens was characterized by electronic scanning electron microscopy and electronic paramagnetic resonance spectroscopy(EPR). The results showed that the size of the SeNPs produced by Bacillus amyloliquefaciens was about 300 nm, with uniform size and high crystallinity. Reactive oxygen species(ROS) and weak environmentally persistent free radicals(EPFRs) were detected on the separated and purified SeNPs. This experiment illustrated the physicochemical properties of SeNPs produced by microorganisms, and evaluated the production of a new type of substance-EPFRs during the production of SeNPs, which provides a new perspective for a comprehensive understanding on microbial transformation of SeNPs.更多还原