【摘要】 目的 研究中性粒细胞胞外诱捕网（NET）对小鼠MC38结直肠癌细胞增殖、迁移能力的影响及其机制。方法 提取小鼠脾脏中性粒细胞，体外用离子霉素刺激获得NET。将NET与MC38细胞共孵育后，采用CCK-8法检测MC38细胞增殖，Transwell^TM和细胞划痕实验检测MC38细胞迁移能力。实时荧光定量PCR检测细胞基质金属蛋白酶2 （MMP2）、 MMP9的mRNA表达，Western blot法检测MC38细胞MMP2、 MMP9和黏着斑激酶（FAK）、磷酸化的FAK蛋白表达。使用小干扰RNA（siRNA）沉默MMP9后，采用前述方法检测NET对MC38细胞增殖、迁移能力的影响及相关分子表达的改变。结果NET促进MC38细胞增殖和迁移能力，并上调MMP9表达、促进FAK的磷酸化。沉默MMP9后，抑制NET对MC38细胞增殖和迁移的促进作用并抑制FAK磷酸化。结论 NET上调MC38细胞MMP9表达，激活FAK信号通路，促进肿瘤细胞增殖和迁移。更多还原

【Abstract】 Objective To investigate how the neutrophil extracellular traps（NETs） affect the proliferation and migration of mouse MC38 colorectal cancer cells and its mechanism. Methods Spleen neutrophils were extracted in mouse, followed by collection of NETs after ionomycin stimulation in vitro. The proliferation of MC38 cell was detected by CCK-8 assay, and migration ability were detected by Transwell^TM and cell scratch assay, after co-incubation with MC38 cells. The mRNA expression of cellular matrix metalloproteinase 2（MMP2） and MMP9 were detected by real-time fluorescence quantitative PCR, and the expression of MMP2, MMP9 and focal adhesion kinase（FAK）, phosphorylated FAK protein were detected by Western blot. After silencing MMP9 using small interfering RNA（siRNA）, the effect of NETs on the proliferation and migration ability of MC38 cells and the altered expression of related molecules were examined by previous approach. ResultsNETs promoted the proliferation and migration of MC38 cells and up-regulated the MMP9 expression and FAK phosphorylation. Silencing MMP9 inhibited the promotion of MC38 proliferation and migration by NETs and suppressed FAK phosphorylation. Conclusion NETs up-regulates MMP9 expression in MC38 cells, activates FAK signaling pathway and promotes tumor cell proliferation and migration.更多还原