【摘要】 目的 观察长链非编码RNA心肌梗死相关转录本（long non coding RNA myocardial infarction associated transcript,lnc RNA MIAT）在子宫颈癌（cervical cancer,CC）中的表达情况，旨在为CC患者的早期诊断及预后评估提供可靠的实验指标。方法 收集2018年1月—2020年12月于枣庄市立医院住院的子宫颈癌患者30例，并对患者进行分期，通过qRT-PCR方法，检测血清、子宫颈癌组织及癌旁正常组织中lnc RNA MIAT的表达水平，并每隔6个月监测患者血清中lnc RNA MIAT的表达水平，分析血清学lnc RNA MIAT的表达水平与子宫颈癌患者临床病理学指标及子宫颈癌生存率的相关性。结果 （1）Ⅲ～Ⅳ期子宫颈癌患者血清中及癌组织中lncRNA MIAT的表达水平高于Ⅰ～Ⅱ期，差异有统计学意义（t=18.567,21.333,P <0.05）。（2）子宫颈癌患者病理特征如肿瘤直径> 4cm，有淋巴结转移、Ⅲ及Ⅳ期、有复发/死亡者中，lncRNAMIAT表达水平显著高于其他项，差异有统计学意义（P <0.05），且lncRNA MIAT表达水平与子宫颈癌患者临床分期指标呈正相关，（r=0.665）。（3）lncRNAMIAT高表达组患者术后2年生存率低于低表达组（χ~2=3.871,P=0.037），每隔6个月表达水平与术后复发率呈正相关（r=0.834），与预后呈负相关（r=-0.789）。结论 子宫颈癌中lncRNA MIAT的表达水平增高，且与子宫颈癌患者的临床分期存在相关性。术后患者血清中lncRNA MIAT的表达水平与预后呈负相关，对患者的预后评估具有潜在价值。更多还原

【Abstract】 Objective To observed the expression on long non coding RNA myocardial infarction associated transcript(lncRNA MIAT) in cervical cancer(CC), in order to provide a reliable experimental index for the early diagnosis and prognosis evaluation reference index of CC patients. Methods A total of 30 patients with cervical cancer who were admitted to Zaozhuang Municipal Hospital from January 2018 to December 2020 were collected, and the patients were staged. By qRTPCR, serum, cervical cancer tissue and adjacent normal tissue were detected for long-chain noncancerous The expression level of myocardial infarction-related transcription factor(MIAT) encoding RNA(lncRNA) was monitored,and the expression level of lncRNA MIAT in serum of patients was monitored every 6 months. The correlation between clinic opathological indicators and cervical cancer survival.Results (1)The expression level of lncRNA MIAT in the serum and cervical cancer tissue of patients with stage Ⅲ-Ⅳ cervical cancer were higher these in stage Ⅰ-Ⅱ patients, and the difference was statistically significant(t =18.567, 21.333, P < 0.05).(2)Case pathological features of cervical cancer patients, such as tumor diameter > 4 cm, lymph node metastasis, stage Ⅲ and Ⅳ, recurrence/death, lnc RNA MIAT expression level was significantly higher than other patients, the difference was statistically significant(P < 0.05) and there was a positive correlation between the expression level of lncRNA MIAT and the clinical stage indicators of cervical cancer patients(r =0.665).(3) The 2-year survival rate of patients in the lncRNA MIAT high expression group was lower than that in low expression groups(χ~2=3.871, P =0.037), the expression level of lnc RNA MIAT in serum every six months was positively correlated with the recurrence rate after surgery(r =0.834), and negatively correlated with the prognosis(r =-0.789). Conclusion The expression level of lncRNA MIAT is increased in cervical cancer, and it is correlated with the clinical stage of cervical cancer patients. The expression level of lncRNA MIAT in serum of postoperative patients was negatively correlated with prognosis, which has potential value for the prognosis evaluation of patients.更多还原