【摘要】 目的 研究丙泊酚麻醉是否损伤大鼠工作记忆编码阶段海马到前额叶皮质神经通路的信息传递。方法12只大鼠中选取可用于研究分析的6只SD大鼠（3月龄），将16通道微电极阵列分别植入大鼠腹侧海马（vHPC）和内侧前额叶皮质（mPFC）。使用Cerebus信息采集系统记录每只大鼠在接受150 mg/kg丙泊酚麻醉前，麻醉后12、24 h执行工作记忆编码阶段任务时，其mPFC和vHPC这两个责任脑区的多通道局部场电位（LFPs）信号，建立vHPC-mFPC网络，分别计算vHPC和mPFC网络的定向传递函数（DTF）和vHPC-mPFC神经通路信息流，定量表征大鼠麻醉前后vHPC-mPFC神经通路信息传递。结果 麻醉后12 h大鼠vHPC和mPFC的平均网络连接强度及vHPC-mPFC信息流均低于麻醉前，而麻醉后24 h vHPC和mPFC的平均网络连接强度及vHPC-mPFC信息流较麻醉前差异均无统计学意义。结论 丙泊酚麻醉短暂损伤工作记忆编码vHPC和m PFC网络连接强度和vHPC-mPFC信息传递。更多还原

【Abstract】 Objective To figure out whether propofol anesthesia impairs information transmission from hippocampal to prefrontal cortex(HPC-PFC) neural pathways during the coding phase of working memory in rats.Methods Among 12 rats,6 SD rats(3 months old) were selected for research analysis,and 16-channel microelectrode arrays were implanted into the medial prefrontal cortex(mPFC) and the ventral hippocampus(vHPC) of rats,respectively.The Cerebus information acquisition system was used to record the working memory coding stage tasks of each rat before and 12 and 24 hours after anesthesia with 150 mg/kg propofol.The multichannel local field potential(LFPs) signals of mPFC and vHPC,two responsible brain regions,were used to establish the vHPC-mFPC network.The directional transfer function(DTF) and the information flow of vHPC-mPFC neural pathway in vHPC and mPFC networks were calculated respectively,and the information transmission of vHPC-mPFC neural pathway before and after anesthesia was quantitatively characterized.Results During working memory encoding,information flow from vHPC to mPFC was significantly reduced 12 h after propofol anesthesia comparison with before propofol anesthesia,but it recovered at 24 h after propofol injection.There were no significant differences in the average vHPC and mPFC network connection strength and vHPC-mPFC information flow of rats between before anesthesia and 24 h after anesthesia.Conclusion These results indicated that working memory impairment induced by propofol may result from disrupting information transmission in vHPC-mPFC network during working memory encoding.更多还原