【摘要】 目的 探究14号染色体开放阅读框174(SAMD15)在胃癌发生发展中的作用以及相应机制。方法 通过数据库分析、免疫蛋白印迹法、荧光定量PCR探究了SAMD15在胃癌临床样本中的表达；通过多种增殖实验及皮下瘤模型检测SAMD15在体内外对胃癌细胞增殖的影响。结果 SAMD15在胃癌组织中高表达。体内及体外实验证明，敲低SAMD15能够抑制人胃腺癌细胞系(AGS)的增殖能力。在机制研究层面，SAMD15通过正向调控AarF结构域激酶1(ADCK1)的表达水平，上调β-catenin以及下游细胞骨髓细胞瘤病毒癌基因(c-Myc)的含量。同时，数据分析表明SAMD15高表达的胃癌患者呈现不良预后。结论 SAMD15能通过ADCK1/β-catenin通路促进胃癌的发生发展，同时其表达水平能够预测部分胃癌患者的生存预后。更多还原

【Abstract】 Objective To investigate the role of SAMD15 in gastric cancer oncogenesis and the possible mechanism.Methods The expression level of SAMD15 in gastric cancer clinical samples was detected by database analysis, Western blotting and fluorescence quantitative PCR.The effect of SAMD15 on gastric cancer cell proliferation was detected in vitro and in vivo.Results SAMD15 was over-expressed in gastric cancer tissues.Both in vitro and in vivo experiments had proved that SAMD15 suppressed the proliferation ability of gastric cancer cells.SAMD15 upregulated β-catenin and its downstream target c-Myc by enhancing the expression level of ADCK1.Meanwhile, database analysis showed that SAMD15 overexpression was associated with poor prognosis in gastric cancer patients.Conclusion SAMD15 promotes gastric cancer oncogenesis through ADCK1/β-catenin pathway, and its expression level may be a predictor for the survival prognosis of some gastric cancer patients.更多还原