【摘要】 目的 基于硫酸钡吸光比浊法，提出一种大蒜废水中大蒜素的检测方法。方法 通过单因素试验和L₉（3⁴）正交试验确定影响测定因素的主次关系和最佳测定条件，通过精密度试验及加标回收试验，验证方法的重现性和准确性。结果 最佳测定参数为波长为415 nm,质量浓度10%BaCl₂溶液5 mL,pH=1,质量浓度2%聚乙烯醇溶液1 mL,稳定时间15 min,吸光度与硫酸根离子质量浓度呈线性关系，y=0.0301x+0.001;相关系数大于0.99。标准曲线的加标回收率为98%～99%,相对标准偏差为1.4%。结论 改进后的硫酸钡吸光比浊法具有较高的重现性和准确性，方法简便快捷，可以满足用户对大蒜废水的快速检测需求。更多还原

【Abstract】 Based on barium sulfate absorption turbidimetry, a method for the determination of garlicin in garlic wastewater was proposed.The primary and secondary relationships during the influencing factors and the optimal determination conditions of the method were determined by the single factor test and L₉（3⁴）orthogonal test.The reproducibility and accuracy of the method were verified by the precision test and spiked recovery test.The optimum experimental conditions are as follows: wavelength=415 nm, pH=1,polyvinyl alcohol=1 mL,BaCl₂=5 mL,and stable time=15 min.Under the optimum experimental conditions, the absorbance（y）of the system was linearly related to the mass concentration（x）of sulfate ion: y=0.030 1x+0.001.The correlation coefficient is greater than 0.99.Recovery rate of spiking is controlled in 98%-99%.The relative standard deviation（RSD）was 1.4%.The improved barium sulfate absorbance turbidimetry has high reproducibility and accuracy, and the method is simple and fast, which can meet the user′s requirements for rapid detection of garlic wastewater.更多还原