【摘要】 目的:为提高顺铂的抗肿瘤效果，检测双氢青蒿素（DHA）与顺铂（CDDP）联合应用时，DHA是否能增强顺铂对人神经母细胞瘤细胞的生长抑制作用。方法:人神经母细胞瘤细胞SH-SY5Y为实验细胞株，分别使用不同浓度的DHA和CDDP单独和同时处理对数期肿瘤细胞；CCK-8法测定细胞的增殖抑制率，流式细胞术检测细胞周期及凋亡率；采用Compusyn软件计算DHA与CDDP的联合指数（CI）。Western Blot实验检测细胞凋亡相关蛋白（Bax、Bcl-2、Cleaved-PARP、PARP、Cleaved-Caspase3、Caspase3）的变化情况。结果:DHA单独用药时药物IC₅₀值为5.794μg/mL,CDDP单独用药时药物IC₅₀值为4.326μg/mL,DHA与CDDP联合用药时药物IC₅₀值为3.253μg/mL,DHA能抑制人神经母细胞瘤细胞增殖并呈浓度依赖性（P<0.05）,促进细胞凋亡（P<0.05）,与CDDP联用后作用更显著，且两药小剂量联用时作用拮抗，大剂量联用时作用协同。结论:DHA体外增强顺铂对人神经母细胞瘤细胞的抗肿瘤效应，提高人神经母细胞瘤细胞对顺铂治疗的敏感性。更多还原

【Abstract】 Objective:To improve the antitumor effect of cisplatin, to test whether dihydroartemisinin（DHA） can enhance the inhibitory effect of cisplatin（CDDP） on the growth of human neuroblastoma cells when combined DHA with and cisplatin.Methods:Human neuroblastoma cell line SH-SY5Y was used as experimental cell line.The logarithmic stage tumor cells were treated with different concentrations of DHA and CDDP separately and simultaneously.Cell proliferation inhibition rate was determined by CCK-8 method, and cell cycle and apoptosis rate were determined by flow cytometry.Compusyn software was used to calculate the combination index（CI） of DHA and CDDP.The changes of apoptosis-related proteins（Bax, Bcl-2,Cleaved-PARP,PARP,Cleaved-Caspase3,Caspase3） were detected by Western Blot.Results:The IC₅₀ value of DHA alone was 5.794 μg/mL,that of CDDP alone was 4.326 μg/mL,and that of DHA combined with CDDP was 3.253 μg/mL.DHA inhibited the proliferation of human neuroblastoma cells in a concentration-dependent manner（P<0.05） and promoted apoptosis（P<0.05）.DHA combined with CDDP had a more significant effect, and the two drugs had an antagonistic effect in low dose and a synergistic effect in high dose.Conclusion:DHA enhances the antitumor effect of cisplatin on human neuroblastoma cells in vitro, and enhances the sensitivity of human neuroblastoma cells to cisplatin therapy.更多还原