【摘要】 随着科学技术的发展与成熟，分子分型技术备受关注，其中，脉冲场凝胶电泳及全基因组测序已成为多种细菌分型的金标准，而基于电泳的分子分型技术、多位点序列分型、多毒力位点序列分型、分子血清分型也各有优缺点和适用范围。该文拟对产单核细胞李斯特菌分子分型技术进行详细阐述，旨在为临床李斯特菌病的暴发流行、同源性分析提供研究方法，为食源性疾病的监测、预警、风险评估及溯源提供参考依据。更多还原

【Abstract】 With the development and maturity of science and technology, molecular typing technology has attracted much attention, among which, pulsed field gel electrophoresis and whole genome sequencing have become the gold standard for a variety of bacterial typing, and electrophoretic-based molecular typing technology, multilocus sequence typing, multi-virulence site sequence typing, and molecular serotyping also have their advantages and disadvantages and applicable ranges. In this paper,the molecular typing technique of Listeria Monocytogenes was described in detail, aiming to provide research methods for the outbreak and homology analysis of clinical Listeria Monocytogenes, and provide reference basis for monitoring, warning, risk assessment, and traceability of foodborne diseases.更多还原