【摘要】 为实现甲醛的高效无害处理，以甲醛为唯一碳源，从天津市某污水处理厂的好氧池活性污泥中分离筛选得到1株高效甲醛降解细菌WY0,研究其生长特性及不同培养温度、接种量、pH值、甲醛质量浓度、NaCl质量浓度以及外加碳氮源等条件对菌株降解甲醛的影响，并运用Three-Half-Order动力学方程拟合菌株在不同质量浓度甲醛下的降解过程。结果表明：通过菌落形态和分子生物学鉴定，将WY0鉴定为马氏无色杆菌(Achromobacter marplatensis)。菌株WY0的最大比增长速率(SGompertz模型)为0.603,在10%接种量、温度25～35℃、pH值4～6、NaCl质量浓度为0～30 g/L的条件下均能高效降解甲醛，能够耐受1 000 mg/L甲醛，Three-Half-Order模型与菌株的甲醛降解过程拟合度良好，R~2均大于0.96。A.marplatensis可在高盐环境下正常降解甲醛，为甲醛废水的生物处理过程提供了新的菌种选择。更多还原

【Abstract】 In order to achieve efficient and harmless treatment of formaldehyde, a highly efficient formaldehyde degrading bacterium WY0 was isolated and screened from the activated sludge of an aerobic tank of a sewage treatment plant in Tianjin by using formaldehyde as the only carbon source, and its growth characteristics and the effects of different culture temperature, pitching rate, pH, formaldehyde mass concentration, NaCl and additional carbon and nitrogen sources on the strain’s formaldehyde degradation were studied. The three-half-order kinetic equation was used to fit the degradation process of strain under different mass concentrations of formaldehyde. The results showed that WY0 was identified as Achromobacter marplatensis by colony morphology and molecular biology identification. The maximum specific growth rate(SGompertz model) of strain WY0 was 0.603. Under the conditions of 10% inoculation amount, temperature 25-35 ℃, pH 4-6, and NaCl mass concentration 0-30 g/L, strain WY0 could efficiently degrade formaldehyde, and could tolerate 1 000 mg/L formaldehyde. The three-half-order model fitted well with the formaldehyde degradation process of the strain, R~2 was greater than 0.96.更多还原