【摘要】 为克服维生素K₂（Vitamin K₂, VK₂）提取工艺有毒溶剂用量大、有毒物质残留等缺点，建立一种高纯度VK₂绿色分离纯化工艺方法。使用天然油剂与甲醇、乙醇等有机试剂分别萃取纳豆芽孢杆菌干菌体内的VK₂,结果表明，中链甘油三酯的萃取效果最好，最大提取量为2.7 mg/g。通过使用7种不同型号的大孔树脂分离中链甘油三酯溶剂相中的VK₂,以及利用静态吸附与解吸附实验，筛选出最优型大孔树脂HC-200S。进一步优化大孔树脂的动态吸附和解吸附工艺，并且绘制大孔树脂的动态解吸附曲线，结果表明：上样液吸附流速为2.0 mL/min、解吸剂为无水乙醇/乙酸丁酯（1∶2,体积比）的混合溶液、解吸剂流速为1.0 mL/min时，HC-200S型大孔树脂对VK₂的吸附和解吸附效果最佳；VK₂纯度较纯化前提升4倍，达到87.8%,回收率达到93.1%。将树脂分离纯化得到的VK₂解吸液经过冷冻结晶和溶剂挥发结晶，分别得到纯度为96.7%和97.1%的VK₂晶体。研究将为VK₂的工业生产和绿色分离纯化提供方法依据，同时也为其他脂溶性维生素的分离纯化提供借鉴。更多还原

【Abstract】 In order to overcome the shortcomings of high purity Vitamin K₂ extraction process, such as large amount of toxic solvent, serious pollution and toxic substance residues, a green separation and purification process of high purity Vitamin K₂ was established. Natural oil, methanol, ethanol and other organic reagents were used to extract VK₂ from Bacillus natto. The results showed that medium chain triglyceride had the best extraction effect, and the maximum extraction amount was 2.7 mg/g. Seven types of macroporous resins were used to separate VK₂ from medium chain triglyceride solvent phase, and the optimal macroporous resin HC-200S was selected by static adsorption and desorption experiments. The dynamic adsorption and desorption process of macroporous resin was optimized, and the dynamic desorption curve of macroporous resin was drawn. The results showed that when the adsorption flow rate of sample solution was 2.0 mL/min, the desorption agent was anhydrous ethanol/butyl acetate mixture solution（1∶2, volume ratio） and the flow rate of desorption agent was 1.0 mL/min, the adsorption and desorption effect of HC-200S macroporous resin on VK₂ was the best. At this time, the purity of vitamin K₂ was 4 times higher than that of the before purification, reaching 87.8%, and the recovery rate reached 93.1%. VK₂ desorption solutions obtained by resin separation and purification were frozen crystallization and solvent volatilization crystallization, respectively, to obtain VK₂ crystals with purity of 96.7% and 97.1%. This study provided a method basis for the industrial production and green separation and purification of VK₂.更多还原