【摘要】 为研究红螯螯虾雌雄间性个体与正常个体之间的差异，对红螯螯虾雌雄间性个体和正常个体的精巢和卵巢运用Illumina Novaseq 6000进行转录组测序，同时借助生物信息学开展功能基因预测和基因表达对比分析。通过测序和组装，共获得83.79 Gb质控碱基，各样品质控碱基均达到6.29 Gb以上，Q30碱基百分比在94.25%以上，组装得到的Unigene个数为53 077,转录本个数为73 588。基因/转录本与6大数据库比对上的比例为18.17%～36.07%。基因表达分析结果表明：样本间共表达的Unigene数为3 543个，不同个体卵巢的表达量相关系数最大，不同个体精巢的表达量相关系数次之，卵巢和精巢之间表达量相关系数最小；正常个体的卵巢和精巢与不同雌性间性个体的精巢和卵巢的基因表达量存在差异，累计差异表达的基因有310个。研究认为，红螯螯虾雌雄间性个体不仅在外部形态上与正常个体存在差异，在性腺的转录水平上也存在着差异，且不同类型的雌雄间性个体与正常个体之间的差异程度不同。更多还原

【Abstract】 In order to study the difference between the intersex and normal individuals, their testis and ovary were sequenced with Illumina Novaseq 6000 in this study, and were analyzed and compared in biological information and gene expression level. With sequencing, 83.79 Gb clean reads were obtained in total, and 6.29 Gb on average, the Q30 bases percentage was more than 94.25%. After assembling, 53 077 Unigenes and 73 588 transcripts were obtained, then they were mapped with the six international data, the mapping ratio was 18.17%-36.07%. The gene expression analyzing indicated that there were 3 543 Unigenes co-expressed, the correlation of expression between ovaries was the greatest, the correlation for testis was followed, the correlation between ovary and testis was the last one. There were differences in the amount of expression gene between normal and intersex individuals, with 310 genes in total. In conclusion, besides differences in morphology between intersex and normal individuals, there were differences in transcription level for gonads, and the level was different for different kinds of intersex individuals.更多还原