【摘要】 目的 探究绣球菌浓缩液（concentrated Sparassis crispa extract, SCE）对黑色素合成和氧化损伤成纤维细胞的影响。方法 以酵母β-葡聚糖为对照,通过检测酪氨酸酶酶活抑制率、黑色素瘤B16细胞内黑色素产生、1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基清除率及人成纤维细胞存活率、细胞内活性氧（reactive oxygen species, ROS）水平和细胞合成胶原蛋白、弹性蛋白的浓度,探讨SCE抑制黑色素合成和成纤维细胞氧化损伤的保护作用。结果 在本研究的测试浓度范围内,SCE对酪氨酸酶活性抑制率为35.0%～79.4%,对黑色素瘤B16细胞黑色素产生抑制率为24.6%～52.1%,并表现出浓度依赖性。同时, SCE能有效清除DPPH自由基,对H₂O₂诱导的人成纤维细胞凋亡具有保护作用,并可降低细胞内ROS水平;与模型组比较, SCE在10～100μg/mL范围内对成纤维细胞合成Ⅲ型胶原蛋白提高了67%～79%、弹性蛋白合成提高了124%～199%,且效果优于酵母β-葡聚糖。结论 SCE具有抑制黑色素合成、保护氧化损伤成纤维细胞和提高成纤维细胞合成胶原蛋白、弹性蛋白的作用,这为其应用于美容功能食品提供依据。更多还原

【Abstract】 Objective To explore the effects of the concentrated Sparassis crispa extract（SCE） on melanin synthesis and oxidatively damaged fibroblasts. Method Yeast β-glucan was chosen as a control, the inhibition rate of tyrosinase activity, melanin synthesis in B16 melanoma cells, 1,1-diphenyl-2-picrylhydrazyl（DPPH） radical clearance rate, human fibroblast survival rate, intracellular reactive oxygen species（ROS） levels, and the concentrations of collagen and elastin synthesized by fibroblasts were investigated to evaluate the inhibitory effect of SCE on melanin synthesis and its protective effect on oxidative damage of fibroblasts. Results The inhibition rates of tyrosinase activity of SCE were 35.0%-79.4% in the range of testing concentrations and the inhibition rates of melanin synthesis in B16 melanoma cells were 24.6%-52.1%, both exhibiting a concentration-dependent manner.Meanwhile, SCE could effectively scavenge DPPH radicals, have a protective effect on H₂O₂-induced apoptosis of human fibroblasts and reduce the levels of intracellular ROS. Furthermore, compared with the model, SCE increased the content of type Ⅲ collagen by 67%-79% and elastin by 124%-199% in fibroblasts in the range of 10-100 μg/mL.Particularly, the improvement effects of SCE were better than those of yeast β-glucan regarding antioxidative actions.Conclusion SCE can inhibit melanin synthesis in B16 melanoma cells, protect fibroblasts from oxidative damages,and improve the synthesis of collagen and elastin in fibroblasts, which provides a basis for its application in cosmetic functional food.更多还原